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1.1.1.428: 4-methylthio 2-oxobutanoate reductase (NADH)

This is an abbreviated version!
For detailed information about 4-methylthio 2-oxobutanoate reductase (NADH), go to the full flat file.

Reaction

(2R)-2-hydroxy-4-(methylsulfanyl)butanoate
+
NAD+
=
4-(methylsulfanyl)-2-oxobutanoate
+
NADH
+
H+

Synonyms

4-methylthio 2-oxobutyrate reductase, 4-methylthio 2-oxobutyric acid reductase, C-terminal binding protein 1, C-terminal-binding protein 1, C-terminal-binding protein 2, CtBP, CTBP1, CtBP2, MTOB reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.428 4-methylthio 2-oxobutanoate reductase (NADH)

Crystallization

Crystallization on EC 1.1.1.428 - 4-methylthio 2-oxobutanoate reductase (NADH)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CtBP1 in complex with 4-(methylsulfanyl)-2-oxobutanoate and NAD+, hanging drop vapor diffusion method, using 200-300 mM magnesium chloride, 0-140 mM sodium formate, 100 mM HEPES pH 7.5, and 2.5 mM NAD+
CtBP2 in complex with 4-(methylsulfanyl)-2-oxobutanoate and NAD+, hanging drop vapor diffusion method, using 200 mM, potassium nitrate, 15-20% PEG 3350, and 100 mM bis-tris-propane pH 7.0
enzyme in complex with phenylpyruvate is crystallized by hanging drop vapor diffusion against a reservoir solution containing 200 mM CaCl2, 100 mM HEPES pH 7.5, and 2.5 mM NAD+. Enzyme in complex with 2-hydroxyimino-3-phenylpropanoic acid is crystallized by vapor diffusion against a reservoir solution containing 75 mM CaCl2, 100 mM HEPES pH 7.5, and 1 mM NADH
-
selenomethionine-substituted enzyme bound to NAD+, using 140 mM sodium formate, 70 mM magnesium acetate, and 100 mM HEPES (pH 7.0)
-