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0.016 - 4.9
2-oxoglutarate
138
D-isocitrate
-
recombinant enzyme, pH 8.0, 30°C
0.0005 - 83.03
DL-isocitrate
additional information
additional information
-
0.016
2-oxoglutarate
kcat less than 0.016 s-1 or equal, mutant enzyme H133Q, at pH 7.5 and 37°C
0.017
2-oxoglutarate
wild type enzyme, at pH 7.5 and 21°C
0.019
2-oxoglutarate
wild type enzyme, at pH 7.5 and 37°C
0.019
2-oxoglutarate
kcat less than 0.019 s-1 or equal, mutant enzyme A134D, at pH 7.5 and 37°C
0.02
2-oxoglutarate
mutant enzyme A134D, at pH 7.5 and 21°C
0.034
2-oxoglutarate
mutant enzyme H133, at pH 7.5 and 21°C
0.128
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 21°C
0.34
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 37°C
0.37
2-oxoglutarate
mutant enzyme R132A, at pH 7.5 and 37°C
0.43
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 21°C
0.45
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 21°C
0.54
2-oxoglutarate
mutant enzyme R132W, at pH 7.5 and 37°C
0.57
2-oxoglutarate
mutant enzyme R132K, at pH 7.5 and 37°C
0.79
2-oxoglutarate
mutant enzyme R132N, at pH 7.5 and 37°C
0.84
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 21°C
1
2-oxoglutarate
mutant enzyme R153C, at pH 7.5 and 25°C
1
2-oxoglutarate
mutant enzyme R153H, at pH 7.5 and 25°C
1.44
2-oxoglutarate
mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C
1.59
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 37°C
1.6
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 37°C
1.6
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
1.87
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
3.2
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
3.3
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C
3.4
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
3.5
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
3.6
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
3.8
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
3.8
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
3.87
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
4.1
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
4.1
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
4.2
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 37°C
4.7
2-oxoglutarate
mutant enzyme R132Q, at pH 7.5 and 37°C
4.9
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
0.0005
DL-isocitrate
Q8X277
mutant R291S/Y390P, pH 8.0, 30°C
0.0013
DL-isocitrate
Q8X277
mutant R291S/K343D/Y390P, pH 8.0, 30°C
0.002
DL-isocitrate
Q8X277
mutant R291S, pH 8.0, 30°C
0.0023
DL-isocitrate
Q8X277
wild-type, pH 8.0, 30°C
0.0025
DL-isocitrate
Q8X277
mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C
0.005
DL-isocitrate
Q8X277
mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C
1.9
DL-isocitrate
-
isoform ICD-1, presence of Zn2+, pH 7.5, 25°C
3.8
DL-isocitrate
-
isoform ICD-1, presence of Mg2+, pH 7.5, 25°C
10.29
DL-isocitrate
with Mg2+, pH 7.5, 50°C
10.92
DL-isocitrate
with Mg2+, pH 7.5, 37°C
19.6
DL-isocitrate
-
isoform ICD-2, presence of Mg2+, pH 7.5, 25°C
43.21
DL-isocitrate
with Mn2+, pH 7.5, 50°C
43.21
DL-isocitrate
with Mn2+, pH 7.5, 37°C
83.03
DL-isocitrate
-
at pH 7.4 and 37°C
0.047
isocitrate
mutant enzyme R132N, at pH 7.5 and 37°C
0.12
isocitrate
mutant enzyme R132H, at pH 7.5 and 21°C
0.18
isocitrate
mutant enzyme R153H, at pH 7.5 and 25°C
0.2
isocitrate
mutant enzyme A134D, at pH 7.5 and 21°C
0.3
isocitrate
mutant enzyme R153C, at pH 7.5 and 25°C
1
isocitrate
mutant enzyme R132G, at pH 7.5 and 21°C
1.21
isocitrate
mutant enzyme R132W, at pH 7.5 and 37°C
1.4
isocitrate
mutant enzyme R100Q, at pH 7.5 and 21°C
1.61
isocitrate
mutant enzyme R132C, at pH 7.5 and 21°C
2.1
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
2.3
isocitrate
mutant enzyme A134D, at pH 7.5 and 37°C
2.4
isocitrate
mutant enzyme R132H, at pH 7.5 and 37°C
2.66
isocitrate
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
3 - 6
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
3.2
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
4
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
4.31
isocitrate
mutant enzyme C3636S, at pH 7.5 and 25°C
4.4
isocitrate
mutant enzyme R132C, at pH 7.5 and 37°C
4.61
isocitrate
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
4.9
isocitrate
mutant enzyme K413Q, at pH 8.0 and 37°C
4.93
isocitrate
wild type enzyme, at pH 7.5 and 25°C
5.6
isocitrate
mutant enzyme R100Q, at pH 7.5 and 37°C
5.6
isocitrate
wild type isoform IDH2, at pH 7.4 and 25°C
7
isocitrate
mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C
7.2
isocitrate
mutant enzyme R132K, at pH 7.5 and 37°C
9.2
isocitrate
mutant enzyme R132Q, at pH 7.5 and 37°C
9.3
isocitrate
mutant enzyme R132G, at pH 7.5 and 37°C
9.4
isocitrate
mutant enzyme H133Q, at pH 7.5 and 21°C
10.4
isocitrate
mutant enzyme R132A, at pH 7.5 and 37°C
11
isocitrate
wild type enzyme, at pH 7.5 and 21°C
15
isocitrate
mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C
15.5
isocitrate
wild type enzyme, at pH 8.0 and 37°C
20
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
20.2
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
22
isocitrate
mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C
23
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
23.1
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
27.5
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
28.7
isocitrate
mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C
31.3
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
31.7
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
32
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
33
isocitrate
pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme
33.4
isocitrate
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
35.4
isocitrate
wild type enzyme, in Tris buffer, at pH 7.8 and 37°C
35.5
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
37
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
37.6
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
38.3
isocitrate
wild type enzyme, in Tris buffer, at pH 8.0 and 37°C
39
isocitrate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
39.2
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C
40
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
40.4
isocitrate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
42
isocitrate
mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C
45
isocitrate
mutant enzyme H133Q, at pH 7.5 and 37°C
50.4
isocitrate
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
51.8
isocitrate
wild type enzyme, at pH 7.5 and 25°C
58.3
isocitrate
-
oxidative decarboxylation
69.88
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
82.47
isocitrate
-
mutant enzyme A741S, at pH 8.0 and 20°C
85
isocitrate
wild type enzyme, at pH 7.5 and 37°C
89.04
isocitrate
-
mutant enzyme E596L, at pH 8.0 and 20°C
102.98
isocitrate
-
at pH 8.0 and 20°C
106.4
isocitrate
40°C, pH 8.0
136.85
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
158.91
isocitrate
-
mutant enzyme L594E, at pH 8.0 and 20°C
255
isocitrate
60°C, pH 8.0
0.0007
NAD+
Q8X277
mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C
0.03
NAD+
mutant D375N, 25°C, pH 7.4
0.38
NAD+
pH 8.5, 55°C, recombinant mutant R322D
0.47
NAD+
mutant K260Q, 25°C, pH 7.4
0.5
NAD+
mutant K260R, 25°C, pH 7.4
0.518
NAD+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
0.76
NAD+
wild-type, 25°C, pH 7.4
1.37
NAD+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+
2.11
NAD+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+
8.9
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
11.7
NAD+
pH 8.0, 25°C, recombinant wild-type enzyme
16.7
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
30.8
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
52.3
NAD+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
56.1
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
62.4
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
96.5
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
113.9
NAD+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
0.0003
NADP+
Q8X277
mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C
0.001
NADP+
Q8X277
mutant R291S/Y390P, pH 8.0, 30°C
0.0024
NADP+
Q8X277
mutant R291S/K343D/Y390P, pH 8.0, 30°C
0.003
NADP+
Q8X277
wild-type, pH 8.0, 30°C
0.004
NADP+
Q8X277
mutant R291S, pH 8.0, 30°C
0.307
NADP+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
1.7
NADP+
mutant K260Q, 25°C, pH 7.4
2
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
2.9
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
3.13
NADP+
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
3.4
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
4
NADP+
-
isoform ICD-1, presence of Mg2+, pH 7.5, 25°C
4.24
NADP+
pH 7.5, 25°C, recombinant mutant R322D, with Mg2+
5.7
NADP+
mutant K260R, 25°C, pH 7.4
5.81
NADP+
wild type enzyme, at pH 7.5 and 25°C
6.36
NADP+
wild type isoform IDH2, at pH 7.4 and 25°C
7.69
NADP+
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
7.9
NADP+
mutant enzyme K413Q, at pH 8.0 and 37°C
8.4
NADP+
mutant D375N, 25°C, pH 7.4
23.2
NADP+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+
23.5
NADP+
wild type enzyme, at pH 8.0 and 37°C
25.36
NADP+
with Mg2+, pH 7.5, 50°C
25.36
NADP+
with Mg2+, pH 7.5, 37°C
27.6
NADP+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+
28.5
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
29.2
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
29.8
NADP+
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
30.3
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
32.2
NADP+
wild-type, 25°C, pH 7.4
33
NADP+
pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme
36.4
NADP+
pH 8.0, 25°C, recombinant wild-type enzyme
37.4
NADP+
-
isoform ICD-2, presence of Mg2+, pH 7.5, 25°C
48.88
NADP+
with Mn2+, pH 7.5, 50°C
48.88
NADP+
with Mn2+, pH 7.5, 37°C
53.1
NADP+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
55.5
NADP+
wild type enzyme, in the presence of Mn2+, at pH 8.2 and 45°C
56
NADP+
at pH 8.5 and 42°C
57.9
NADP+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
60.1
NADP+
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
72
NADP+
pH 7.5, 25°C, recombinant wild-type enzyme, with Mg2+
84.29
NADP+
-
at pH 7.4 and 37°C
138
NADP+
-
recombinant enzyme, pH 8.0, 30°C
additional information
additional information
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
-
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
turnover numbers of wild-type and chimeric enzymes
-
additional information
additional information
-
turnover numbers of wild-type and chimeric enzymes
-