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0.004
-
with (2S,3s)-butane-2,3-diol as substrate, at pH 7.4 and 30°C
0.005
-
with pentane-1,2-diol as substrate, at pH 7.4 and 30°C
0.007
-
with butanone as substrate, at pH 7.4 and 30°C
0.01
-
with pentane-2,3-dione as substrate, at pH 7.4 and 30°C
0.012
-
with hexane-2,5-dione as substrate, at pH 7.4 and 30°C
0.019
-
with pentane-2,4-dione as substrate, at pH 7.4 and 30°C
0.044
-
with n-butyöaödehyde as substrate, at pH 7.4 and 30°C
0.048
-
with butane-1,2-diol as substrate, at pH 7.4 and 30°C
0.084
-
with diacetyl as substrate, at pH 7.4 and 30°C
0.093
-
with propane-1,2-diol as substrate, at pH 7.4 and 30°C
0.1
wild type enzyme, using 1 mM NADPH as cosubstrate
0.155
pH 7.4, 30°C, substrate propane-1,2-diol
0.24
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADPH
0.257
-
with 1-hydroxy-2-butanone as substrate, at pH 7.4 and 30°C
0.331
pH 7.4, 30°C, substrate 1-hydroxy-2-butanone
0.383
pH 7.4, 30°C, substrate butane-1,2-diol
0.48
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH
0.732
-
with 1,3-dihydroxyacetone as substrate, at pH 7.4 and 30°C
0.76
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADPH
0.82
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADPH
1.056
-
with (2R,3R)-butane-2,3-diol as substrate, at pH 7.4 and 30°C
1.16
purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH
1.179
pH 7.4, 30°C, substrate (2R,3S)-butane-2,3-diol
1.193
-
with (R)-acetoin as substrate, at pH 7.4 and 30°C
1.3
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH
1.346
pH 7.4, 30°C, substrate 2,3-pentanedione
1.538
pH 7.4, 30°C, substrate (2R,3R)-butane-2,3-diol
1.71
purified recombinant enzyme, pH 7.0, 30°C, substrates formaldehyde and NADH
1.94
purified recombinant enzyme, pH 7.0, 30°C, substrates propionaldehyde and NADH
1.949
pH 7.4, 30°C, substrate rac acetoin
10.2
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH
10.48
-
crude enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C
117.95
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH
13.7
mutant enzyme E221S, using 1 mM NADPH as cosubstrate
2.154
pH 7.4, 30°C, substrate diacetyl
2.47
purified recombinant enzyme, pH 7.0, 30°C, substrates hexaldehyde and NADH
2.72
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH
2.74
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH
20.75
-
purified enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C
3.44
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADPH
35.55
-
diacetyl, enzyme 2
35.6
-
diacetyl, enzyme 1
4.1
mutant enzyme E221S, using 1 mM NADH as cosubstrate
4.65
-
purified native enzyme, pH 7.0, 30°C
4.8
mutant enzyme E221S/I222R, using 1 mM NADH as cosubstrate
4.95
purified recombinant enzyme, pH 7.0, 30°C, substrates furfural and NADH
54.7
mutant enzyme E221S/I222R, using 1 mM NADPH as cosubstrate
6.1
mutant enzyme E221S/I222R/A223S, using 1 mM NADH as cosubstrate
6.27
purified recombinant enzyme, pH 7.0, 30°C, substrates glycolaldehyde and NADH
78.58
purified recombinant enzyme, pH 7.0, 30°C, substrates acetaldehyde and NADH
92
wild type enzyme, using 1 mM NADH as cosubstrate
93.2
mutant enzyme E221S/I222R/A223S, using 1 mM NADPH as cosubstrate
additional information
-
effects of growth substrate on enzyme activity in vivo, overview
additional information
-
effects of growth substrate on enzyme activity in vivo, overview