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1.1.1.378: L-rhamnose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about L-rhamnose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

L-rhamnose
+
NAD(P)+
=
L-Rhamnono-1,4-lactone
 +
NAD(P)H
 +
H+

Synonyms

AvLRA1, AvRhaDH, EAT09360, L-rhamnose 1-dehydrogenase, LRA1, NAD(P)+-dependent L-rhamnose-1-dehydrogenase, RhaDH, SpLRA1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.378 L-rhamnose 1-dehydrogenase [NAD(P)+]

Engineering

Engineering on EC 1.1.1.378 - L-rhamnose 1-dehydrogenase [NAD(P)+]

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D200A
-
site-directed mutagenesis, the mutant shows 16% activity with L-rhamnose compared to wild-type
D200H
-
site-directed mutagenesis, the mutant shows 22% activity with L-rhamnose compared to wild-type
F99A
-
site-directed mutagenesis, the mutant is inactive
F99Y
-
site-directed mutagenesis, the mutant is inactive
I196A
-
site-directed mutagenesis, the mutant is inactive
Q156A
-
site-directed mutagenesis, the mutant is almost inactive
R15T
-
site-directed mutagenesis, the mutant of AvRhaDH shows a Km value for NADP+ decreased by 116fold, which increases the ratio of NADP+ to NAD+ (0.188) 157fold, from that of the wild-type enzyme (18.5)
S37H
-
site-directed mutagenesis, the mutant shows an altered ratio of NADP+ to NAD+ (0.101) compared to that of the wild-type enzyme (18.5)
T191F
-
site-directed mutagenesis, the mutant shows 3.9% activity with L-rhamnose compared to wild-type