1.1.1.378: L-rhamnose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about L-rhamnose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

Synonyms

AvLRA1, AvRhaDH, EAT09360, L-rhamnose 1-dehydrogenase, LRA1, NAD(P)+-dependent L-rhamnose-1-dehydrogenase, RhaDH, SpLRA1

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.378 L-rhamnose 1-dehydrogenase [NAD(P)+]

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Results	in table
30	AA Sequence
1	Cloned(Commentary)
9	Cofactor
1	Crystallization (Commentary)
5	General Information
30	kcat/KM [mM/s]
30	KM Value [mM]
1	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
6	Organism
7	Pathway
2	pH Optimum
9	Protein Variants
2	Purification (Commentary)
2	Reaction
5	Reference
11	Specific Activity [micromol/min/mg]
24	Substrates and Products (Substrate)
3	Subunits
11	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
30	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.1.1.378 - L-rhamnose 1-dehydrogenase [NAD(P)+]

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme in ligand-free, NAD+-bound, NADP+-bound, and L-rhamnose- and NAD+-bound forms, sitting drop vapor diffusion method, mixing of 500 nl of 10 mg/ml protein in 20 mM Tris/HCl, pH 8.0, and 150 mM NaCl, with 500 nl of reservoir olution containing 100 mM Tris/HCl, pH 8.5, 200 mM Li2SO4, and 25% w/v PEG 3350, with or without coenzyme, for the apoenzyme and for the NADP+-bound form,, and 100 mM Tris/HCl, pH 8.0, 200 mM NaCl, 24.5% w/v PEG 3350, and 3% v/v glycerol only for the NAD+-/L-rhamnose-bound form, equilibration against 0.07 ml of reservoir solution, at 20°C, X-ray diffraction structure determination and analysis at 1.9, 2.1, 2.4, and 1.6 A resolution, respectively, molecular replacement using the structure of 3-oxoacyl-(acyl carrier protein) reductase from Bacillus anthracis (PDB ID 2UVD) as the search model, modeling

Azotobacter vinelandii

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