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1.1.1.366: L-idonate 5-dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about L-idonate 5-dehydrogenase (NAD+), go to the full flat file.

Reaction

L-Idonate
+
NAD+
=
5-Dehydro-D-gluconate
+
NADH
+
H+

Synonyms

GluE, L-IdnDH, L-idonate 5-dehydrogenase, L-idonate dehydrogenase, LidnDH, NAD+ requiring L-idonate 5-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.366 L-idonate 5-dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.366 - L-idonate 5-dehydrogenase (NAD+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) pLysS cells
-
gene gluE, clustered with gene gluD, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains ATCC 90845 and modified CEN.PK2, subcloning in Escherichia coli strain TOP10
-
gene LidnDH, DNA library construction from a single pool of six samples, DNA sequence determination and comparisons, quantitative real-time PCR enzyme expression analysis in different regional samples of cv. Cabernet Sauvignon berries
IdnDh gene, quantitative expression analysis on transcriptional and translational levels in fruits of different stages