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3.33
3alpha-androstanediol
isoform AKR1C4, at pH 7.4 and 25°C
1.8
4-androstenedione
pH 7.4, 37°C
0.959 - 2.9
4-hydroxyandrostenedione
-
0.943 - 1.63
4-hydroxytestosterone
-
20.72
5alpha-androstane-3,17-dione
isoform AKR1C4, at pH 7.4 and 25°C
10.3 - 135
5alpha-androstane-3alpha,17beta-diol
1.623 - 28.07
5alpha-dihydrotestosterone
53.3 - 101.7
5beta-Androstan-3alpha-ol-17-one
191667
androstenol
reombinant His-tagged enzyme, pH 10.5, 25°C
0.51 - 178571
androsterone
28.3 - 46.7
chenodeoxycholic acid
0.00015 - 3.3
cholic acid
0.00044 - 0.94
Dehydrocholic acid
8.8 - 14.2
deoxycholic acid
0.75 - 22.7
dihydrotestosterone
21.7 - 31.7
lithocholic acid
4.167 - 18.4
pregn-4-en-3,20-dione
0.96
testosterone
pH 7.4, 37°C
additional information
additional information
Kcat/Km-value for the 17beta-oxidation of testosterone is 0.183 1/sec*mM, and Kcat/Km-value for the 17beta-reduction of androstenedione is 0.817 1/sec*mM
-
1
(R)-tetralol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.17
(R)-tetralol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.2
(R)-tetralol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.68
(S)-indan-1-ol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1
(S)-indan-1-ol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1
(S)-indan-1-ol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.2
(S)-tetralol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.5
(S)-tetralol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.83
(S)-tetralol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.54
2-decalol
pH 10.5, 10°C
3.7
2-decalol
reombinant His-tagged enzyme, pH 10.5, 25°C
26.88
2-decalol
pH 10.5, 40°C
0.959
4-hydroxyandrostenedione
pH 7.4, 37°C
-
2.25
4-hydroxyandrostenedione
pH 7.4, 37°C
-
2.9
4-hydroxyandrostenedione
pH 7.4, 37°C
-
0.943
4-hydroxytestosterone
pH 7.4, 37°C
-
1.29
4-hydroxytestosterone
pH 7.4, 37°C
-
1.63
4-hydroxytestosterone
pH 7.4, 37°C
-
10.3
5alpha-androstane-3alpha,17beta-diol
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
96.7
5alpha-androstane-3alpha,17beta-diol
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
135
5alpha-androstane-3alpha,17beta-diol
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.623
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant mutant V54L
9.77
5alpha-dihydrotestosterone
isoform AKR1C4, at pH 7.4 and 25°C
28.07
5alpha-dihydrotestosterone
pH 7.4, 37°C, recombinant wild-type enzyme
53.3
5beta-Androstan-3alpha-ol-17-one
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
96.7
5beta-Androstan-3alpha-ol-17-one
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
101.7
5beta-Androstan-3alpha-ol-17-one
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.51
androsterone
pH 10.5, 40°C
4.6
androsterone
isoform AKR1C4, at pH 7.4 and 25°C
70
androsterone
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
86.7
androsterone
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
105
androsterone
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
178571
androsterone
reombinant His-tagged enzyme, pH 10.5, 25°C
28.3
chenodeoxycholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
45
chenodeoxycholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
46.7
chenodeoxycholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.00015
cholic acid
-
recombinant His-tagged mutant S114A/Y153F, pH 8.0, 37°C
0.0005
cholic acid
-
recombinant His-tagged mutant K157A, pH 8.0, 37°C
0.0013
cholic acid
-
recombinant His-tagged mutant Y153F, pH 8.0, 37°C
0.0014
cholic acid
-
recombinant His-tagged mutant S114A, pH 8.0, 37°C
0.411
cholic acid
-
recombinant His-tagged wild-type enzyme, pH 8.0, 37°C
1
cholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
1.2
cholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
3.3
cholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.015
Cyclohexanol
pH 10.5, 40°C
0.1
Cyclohexanol
reombinant His-tagged enzyme, pH 10.5, 25°C
0.00044
Dehydrocholic acid
-
recombinant His-tagged mutant Y153F, pH 8.0, 37°C
0.0037
Dehydrocholic acid
-
recombinant His-tagged mutant S114A, pH 8.0, 37°C
0.94
Dehydrocholic acid
-
recombinant His-tagged wild-type enzyme, pH 8.0, 37°C
8.8
deoxycholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
9
deoxycholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
14.2
deoxycholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
0.75
dihydrotestosterone
pH 7.4, 37°C
1.43
dihydrotestosterone
pH 7.4, 37°C
1.7
dihydrotestosterone
pH 7.4, 37°C
2.98
dihydrotestosterone
pH 7.4, 37°C
22.7
dihydrotestosterone
pH 7.5, 37°C
21.7
lithocholic acid
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
23.3
lithocholic acid
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
31.7
lithocholic acid
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
166.7
NAD+
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
283.3
NAD+
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
583.3
NAD+
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
23.3
NADP+
-
wild type enzyme, in 0.1 M potassium phosphate, pH 7.4, at 25°C
28
NADP+
mutant F217S, pH 7.4, 25°C
42
NADP+
mutant N272T, pH 7.4, 25°C
50
NADP+
wild-type, pH 7.4, 25°C
266.7
NADP+
-
mutant enzyme H216F, in 0.1 M potassium phosphate, pH 7.4, at 25°C
500
NADP+
-
mutant enzyme H216Y, in 0.1 M potassium phosphate, pH 7.4, at 25°C
9
NADPH
wild-type, pH 7.4, 25°C
11
NADPH
mutant N272T, pH 7.4, 25°C
27
NADPH
mutant F217S, pH 7.4, 25°C
4.167
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant wild-type enzyme
18.4
pregn-4-en-3,20-dione
pH 7.4, 37°C, recombinant mutant V54L