1.1.1.346: 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming), go to the full flat file.

Reaction

2-dehydro-L-gulonate
+
NADP+
=
2,5-didehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconate reductase I, 2,5-diketo-D-gluconate reductase II, 2,5-diketo-D-gluconate-reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-D-gluconic acid reductase A, 2,5-DKG reductase, 2,5-DKGR A, 2,5-DKGR B, 2,5-DKGRA, 2,5DKGR, 25DKG reductase, 25DKGR-A, 2KR, AKR5C, CTATCC11996_22452, dkgA, dkgB, Dkr, YqhE, YqhE reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

Temperature Stability

Temperature Stability on EC 1.1.1.346 - 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 38
-
temperature stability of isoform 2,5-diketo-D-gluconate reductase I is measured by storing the enzyme in 0.1 M Tris-HC1 buffer (pH 7.0) for 1 h. The activity of the enzyme decreases gradually with temperature from 0°C to 27°C, falls rapidly above 27°C, and is lost completely at about 38°C. The remaining activity at 27°C is about 77% of that at 0°C
40 - 80
-
after approximately 10 min of deactivation, enzyme activity attains a constant value, independently of deactivation temperature (40-80°C). This value corresponds to the 30% of native activity