1.1.1.346: 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming), go to the full flat file.

Reaction

2-dehydro-L-gulonate
+
NADP+
=
2,5-didehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconate reductase I, 2,5-diketo-D-gluconate reductase II, 2,5-diketo-D-gluconate-reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-D-gluconic acid reductase A, 2,5-DKG reductase, 2,5-DKGR A, 2,5-DKGR B, 2,5-DKGRA, 2,5DKGR, 25DKG reductase, 25DKGR-A, 2KR, AKR5C, CTATCC11996_22452, dkgA, dkgB, Dkr, YqhE, YqhE reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

Specific Activity

Specific Activity on EC 1.1.1.346 - 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0052
-
crude extract, at pH 6.4 and 25°C
0.0276
-
crude extract, at pH 6.4 and 25°C
0.07
Brevibacterium ketosoreductum
-
crude extract, at pH 6.4 and 30°C
0.188
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in pH-regulated cultivation, pH 6.5, 22°C
0.191
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C
0.232
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C
0.243
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C
0.264
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in pH-regulated cultivation, pH 6.5, 22°C
0.308
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in pH-regulated cultivation, pH 6.5, 22°C
1.967
-
after 71.03fold purification, at pH 6.4 and 25°C
3.38
-
after 650fold purification, at pH 6.4 and 25°C
6.3
-
purified enzyme, at pH 7.0 and 30°C
22.2
Brevibacterium ketosoreductum
-
after 317fold purification, at pH 6.4 and 30°C