1.1.1.346: 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming), go to the full flat file.

Reaction

2-dehydro-L-gulonate
+
NADP+
=
2,5-didehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconate reductase I, 2,5-diketo-D-gluconate reductase II, 2,5-diketo-D-gluconate-reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-D-gluconic acid reductase A, 2,5-DKG reductase, 2,5-DKGR A, 2,5-DKGR B, 2,5-DKGRA, 2,5DKGR, 25DKG reductase, 25DKGR-A, 2KR, AKR5C, CTATCC11996_22452, dkgA, dkgB, Dkr, YqhE, YqhE reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.346 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

Engineering

Engineering on EC 1.1.1.346 - 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F22Y
-
the mutation causes a 2.5fold decrease in Km for 2,5-didehydro-D-gluconate whereas the value of kcat remains essentially unchanged
F22Y/A272G
-
substrate-binding pocket double mutant with decreased kcat value for NADPH compared to the wild type enzyme
F22Y/K232G/R235G/R238H/A272G
-
mutant with wild type kcat value for NADPH
F22Y/K232G/R235T/R238H/A272G 420
-
mutant with decreased kcat value for NADPH compared to the wild type enzyme
F22Y/K232G/R238H/A272G
K232G/R238H
-
mutant with decreased kcat value for NADPH compared to the wild type enzyme
K233G
-
the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme
K233H
-
the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity
K233M
-
the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme
K233Q
-
the mutant shows wild type NADPH activity and increased NADH activity
K233R
-
the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity
K233S
-
the mutant shows wild type NADPH activity and increased NADH activity compared to the wild type enzyme
K233T
-
the mutant shows wild type NADPH activity and no NADH activity
Q192R
-
the mutation primarily affects the kcat parameter toward the 2,5-didehydro-D-gluconate substrate, increasing its value approximately 2.5fold, whereas Km is relatively unaffected, or increases slightly
R235C
-
the mutant shows wild type NADPH activity and no NADH activity
R235D
-
the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity
R235E
-
the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity
R235G
-
the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme
R235H
-
the mutant shows wild type NADPH activity and no NADH activity
R235M
-
the mutant shows wild type NADPH activity and no NADH activity
R235N
-
the mutant shows wild type NADPH activity and no NADH activity
R235Q
-
the mutant shows wild type NADPH activity and no NADH activity
R235S
-
the mutant shows wild type NADPH activity and no NADH activity
R235T
-
the mutant shows wild type NADPH activity and increased NADH activity
R235Y
-
the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity
R238D
-
the mutant shows no activity with NADPH and NADH
R238E
-
the mutant shows no activity with NADPH and increased NADH activity compared to the wild type enzyme
R238F
-
the mutant shows wild type NADPH activity and no NADH activity
R238G
-
the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity
R238H
-
the mutant shows wild type NADPH activity and increased NADH activity
R238N
-
the mutant shows reduced NADPH activity and no NADH activity
R238Q
-
the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity
R238Y
-
the mutant shows reduced NADPH activity and increased NADH activity ompared to the wild type enzyme
S233E
-
the mutant shows no activity with NADPH and NADH
S233K
-
the mutant shows no activity with NADPH and NADH
S233M
-
the mutant shows no activity with NADPH and NADH
S233N
-
the mutant shows no activity with NADPH and NADH
S233T
-
the mutant shows wild type NADPH activity and no NADH activity
S233V
-
the mutant shows no activity with NADPH and NADH
V234D
-
the mutant shows wild type NADPH activity and no NADH activity
V234E
-
the mutant shows wild type NADPH activity and no NADH activity
V234I
-
the mutant shows wild type NADPH activity and no NADH activity
V234M
-
the mutant shows wild type NADPH activity and no NADH activity
V234M/R235C
-
the mutant shows wild type NADPH activity and no NADH activity
V234N
-
the mutant shows wild type NADPH activity and no NADH activity
V234Q
-
the mutant shows wild type NADPH activity and no NADH activity
V234S
-
the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity
additional information