1.1.1.313: sulfoacetaldehyde reductase (NADPH)
This is an abbreviated version!
For detailed information about sulfoacetaldehyde reductase (NADPH), go to the full flat file.
Reaction
Synonyms
isethionate formation, reductase D, IsfD, IsfD2, NADPH-dependent sulfoacetaldehyde reductase
ECTree
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Crystallization
Crystallization on EC 1.1.1.313 - sulfoacetaldehyde reductase (NADPH)
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purified His-tagged enzyme IsfD in a ternary complex with NADPH and isethionate, hanging drop vapor diffusion method, mixing of protein in 10 mM HEPES, pH 7.4, 50 mM KCl, 1 mM TCEP (tris(2-carboxyethyl)phosphine), with reservoir solution containing 0.2 M ammonium acetate, 0.1 M sodium citrate, pH 5.5, 30% w/v PEG 4000, 5 mM NADPH, and 0.4 M isethionate, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement using 2NWQ as a search model, structure comparisons, overview