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1.1.1.31: 3-hydroxyisobutyrate dehydrogenase

This is an abbreviated version!
For detailed information about 3-hydroxyisobutyrate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.31

Reaction

3-hydroxy-2-methylpropanoate
+
NAD+
=
2-Methyl-3-oxopropanoate
+
NADH
+
H+

Synonyms

3-HIBADH, 3-hydroxyisobutyrate dehydrogenase, 3-hydroxyisobutyrate dehydrogenase-I, 3-hydroxyisobutyric acid dehydrogenase, 3HIBDH-IV, 3HIBDHI, At4g20930, AtHDH1, beta-hydroxyisobutyrate dehydrogenase, HDH1, HIBADH, HIBDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.31 3-hydroxyisobutyrate dehydrogenase

Engineering

Engineering on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D244A
the mutant shows strong reduction in the activity compared to the wild type enzyme
D275A
the mutant shows a marginal loss of activity compared to the wild type enzyme
F236A
the mutant shows reduction in the activity compared to the wild type enzyme
N172A
the mutant shows dramatic reduction in the activity compared to the wild type enzyme
T207A
the mutant shows reduction in the activity compared to the wild type enzyme
W211A
the mutant shows dramatic reduction in the activity compared to the wild type enzyme
D244A
-
the mutant shows strong reduction in the activity compared to the wild type enzyme
-
D275A
-
the mutant shows a marginal loss of activity compared to the wild type enzyme
-
N172A
-
the mutant shows dramatic reduction in the activity compared to the wild type enzyme
-
D33R
-
higher affinity to cofactor NADP+ than to natural cofactor NAD+