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1.1.1.307: D-xylose reductase [NAD(P)H]

This is an abbreviated version!
For detailed information about D-xylose reductase [NAD(P)H], go to the full flat file.

Word Map on EC 1.1.1.307

Reaction

xylitol
+
NAD(P)+
=
D-xylose
+
NAD(P)H
+
H+

Synonyms

AKR2B5, ALR2, CTHT_0056950, CtXR, dsXR, dual specific xylose reductase, KmXYL1, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent D-xylose reductase-like protein, NAD(P)H-dependent XR, NAD(P)H-dependent xylose reductase, NAD(P)H-linked xylose reductase, NADH/NADPH-xylose reductase, NADP-dependent xylose reductase, PsXR, PsXYL1, SaXYL1, SpXYL1.1, SsXR, Texr, XR, XYL1, xylose reductase, XylR, XyrA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.307 D-xylose reductase [NAD(P)H]

Purification

Purification on EC 1.1.1.307 - D-xylose reductase [NAD(P)H]

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
recombinant His-tagged enzyme 25.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to near homogeneity
Thermochaetoides thermophila
recombinant protein
yeast cell extract is fractionated by biomimetic chromatography using the dye HE-3B (reactive Red 120) as pseudoaffinity ligand coupled to Sepharose CL-4B. In the second step, high-resolution anion-exchange chromatography using Mono Q yields purified isoform ALR2 in overall yieldof 62%
-