1.1.1.30: 3-hydroxybutyrate dehydrogenase

This is an abbreviated version!
For detailed information about 3-hydroxybutyrate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.30

Reaction

(R)-3-hydroxybutanoate
+
NAD+
=
acetoacetate
+
NADH
+
H+

Synonyms

3-D-hydroxybutyrate dehydrogenase, 3-HBDH, 3-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase 2, 3-hydroxybutyrate dehydrogenase-2, 3HBDH, acetoacetyl-CoA reductase, BDH, BDH1, BDH2, BDH3, BdhA, beta-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, beta-hydroxybutyric dehydrogenase, D(-)-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, DHRS6, HBD, HBDH, hydroxybutyrate oxidoreductase, More, NAD-beta-hydroxybutyrate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.30 3-hydroxybutyrate dehydrogenase

Cloned

Cloned on EC 1.1.1.30 - 3-hydroxybutyrate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13
DNA and amino acid sequence determination and analysis
-
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3)
efficiently expressed in Escherichia coli cells harboring pHBDH11
enzyme expression in Escherichia coli strain DH1
enzyme expression in Escherichia coli strain XL-1 Blue
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XL1-Blue cells
expression in Escherichia coli
expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
-
functional co-expression with acetoacetyl-CoA thiolase and PHB synthase in Escherichia coli, recombinant production of poly(3-hydroxybutanoate) granules, overview
-
His-tagged 3-hydroxybutyrate dehydrogenase, expressed in Escherichia coli
-
mature form, expressed in Spodoptera frugiperda, SF9 cells
-
wild-type and mutants expressed in Escherichia coli XL1Blue harbouring pHBDH11. Wild-type HBDH and mutants containing an N-terminal His-tag expressed in Escherichia coli XL1Blue using pQE30 as a vector