1.1.1.295: momilactone-A synthase

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For detailed information about momilactone-A synthase, go to the full flat file.

Reaction

Synonyms

3beta-hydroxy-9beta-primara-7,15-dien-19,6beta-olide dehydrogenase, AK103462 protein, CpMAS, momilactone A synthase, MS1, MS2, Os04g0179200, OsMAS, OsMAS2

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.295 momilactone-A synthase

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Results	in table
8	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
10	Cofactor
2	Expression
5	General Information
4	kcat/KM [mM/s]
4	KM Value [mM]
1	Localization
4	Natural Substrates/ Products (Substrates)
8	Organism
3	Pathway
1	pH Optimum
1	pH Range
1	Protein Variants
1	Purification (Commentary)
1	Reaction
7	Reference
2	Source Tissue
13	Substrates and Products (Substrate)
13	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Turnover Number [1/s]

Engineering

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Engineering on EC 1.1.1.295 - momilactone-A synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

Calohypnum plumiforme

A0A6F8PFR7

successful momilactone biosynthetic pathway reconstitution in Nicotiana benthamiana expressing the biosynthetic gene cluster (BGC), transient coexpression of CpMAS with CpDTC1/HpDTC1, CpCYP970A14, and CpCYP964A1 genes encoding diterpene cyclase 1, pimaradiene oxidase 1, and pimaradiene oxidase 2, respectively. The potential enzymatic activity of momilactone A synthesis from 3OH-syn-pimaradienolide exists endogenously in Nicotiana benthamiana leaves, based on in vitro assays with crude protein and in planta feeding assays. Eventually, coexpression of CpMAS with other clustered genes increases the production of momilactone A, and in planta conversion of 3OH-syn-pimaradienolide to momilactone A is apparently enhanced by CpMAS expression in Nicotiana benthamiana as compared to that in the vector control plant

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