1.1.1.294: chlorophyll(ide) b reductase
This is an abbreviated version!
For detailed information about chlorophyll(ide) b reductase, go to the full flat file.
Word Map on EC 1.1.1.294
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1.1.1.294
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stay-green
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oxygenase
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light-harvesting
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pheophorbide
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7-hydroxymethyl
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dark-induced
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thylakoids
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ryegrass
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lhcii
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lolium
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perenne
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perennial
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etioplasts
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grana
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pheophytinase
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pao
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chlorophyll-binding
- 1.1.1.294
-
stay-green
- oxygenase
-
light-harvesting
- pheophorbide
-
7-hydroxymethyl
-
dark-induced
-
thylakoids
-
ryegrass
- lhcii
-
lolium
-
perenne
-
perennial
- etioplasts
-
grana
-
pheophytinase
- pao
-
chlorophyll-binding
Reaction
Synonyms
BoNYC1, CBR, Chl b reductase, chlorophyll b reductase, chlorophyll(ide) b reductase, LpNYC1, More, NOL, NON-YELLOW COLORING 1, NON-YELLOW COLORING1, NYC-like, NYC1, NYC1-LIKE
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Cloned
Cloned on EC 1.1.1.294 - chlorophyll(ide) b reductase
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coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3
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fusion of the region encoding the putative transit peptide of NOL to the green fluorescent protein (GFP) gene and introduced into the epidermal cells of Allium cepa by particle bombardment
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gene nol, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview
gene NYC1, cloned using the RACE-PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semiquantitative and quantitative RT-PCR enzyme expression analysis, transient overexpression of LpNYC1 in wild-type Nicotiana benthamiana under control of CaMV 35S promoter and in Arabidopsis thaliana nyc1 null mutant strain AGL1, both via Agrobacterium tumefaciens transfection, recombinant expression of GFP-tagged enzyme NYC1 in Lolium perenne protoplasts
gene nyc1, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview
genes nyc1 and nol, the NYC1 promoter contains a potential abscisic acid-responsive element, semiquantitative reverse transcription-PCR expression analysis of isozymes
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