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1.1.1.294: chlorophyll(ide) b reductase

This is an abbreviated version!
For detailed information about chlorophyll(ide) b reductase, go to the full flat file.

Word Map on EC 1.1.1.294

Reaction

71-hydroxychlorophyllide a
+
NAD(P)+
=
chlorophyllide b
+
NAD(P)H
+
H+

Synonyms

BoNYC1, CBR, Chl b reductase, chlorophyll b reductase, chlorophyll(ide) b reductase, LpNYC1, More, NOL, NON-YELLOW COLORING 1, NON-YELLOW COLORING1, NYC-like, NYC1, NYC1-LIKE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.294 chlorophyll(ide) b reductase

Cloned

Cloned on EC 1.1.1.294 - chlorophyll(ide) b reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3
-
fusion of the region encoding the putative transit peptide of NOL to the green fluorescent protein (GFP) gene and introduced into the epidermal cells of Allium cepa by particle bombardment
-
gene nol, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview
gene NYC1, cloned using the RACE-PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semiquantitative and quantitative RT-PCR enzyme expression analysis, transient overexpression of LpNYC1 in wild-type Nicotiana benthamiana under control of CaMV 35S promoter and in Arabidopsis thaliana nyc1 null mutant strain AGL1, both via Agrobacterium tumefaciens transfection, recombinant expression of GFP-tagged enzyme NYC1 in Lolium perenne protoplasts
gene nyc1, DNA and amino acid determination and anaylsis, phylogenetic tree, promoter analysis, semiquantitative RT-PCR expression analysis, overview
gene NYC1, real-time PCR enzyme expression analysis
-
genes nyc1 and nol, the NYC1 promoter contains a potential abscisic acid-responsive element, semiquantitative reverse transcription-PCR expression analysis of isozymes
-
positional cloning of nyc1
-