1.1.1.290: 4-phosphoerythronate dehydrogenase

This is an abbreviated version, for detailed information about 4-phosphoerythronate dehydrogenase, go to the full flat file.

Reaction

4-phospho-D-erythronate
+
NAD+
=
(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate
+
NADH
+
H+

Synonyms

4-O-phosphoerythronate dehydrogenase, D-erythronate-4-phosphate dehydrogenase, erythronate-4-phosphate dehydrogenase, More, pdx gene product, PDXB, PdxB 4PE dehydrogenase, RdxB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.290 4-phosphoerythronate dehydrogenase

Purification

Purification on EC 1.1.1.290 - 4-phosphoerythronate dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography
-
Ni-agarose column chromatography; recombinant His-tagged PdxB from Escherichia coli stran BL21(DE3) by nickel affinity chromatography and ultrafiltration
-