1.1.1.290: 4-phosphoerythronate dehydrogenase

This is an abbreviated version!
For detailed information about 4-phosphoerythronate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.290

Reaction

4-phospho-D-erythronate
+
NAD+
=
(3R)-3-hydroxy-2-oxo-4-phosphooxybutanoate
+
NADH
+
H+

Synonyms

4-O-phosphoerythronate dehydrogenase, D-erythronate-4-phosphate dehydrogenase, erythronate-4-phosphate dehydrogenase, More, pdx gene product, PDXB, PdxB 4PE dehydrogenase, RdxB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.290 4-phosphoerythronate dehydrogenase

Crystallization

Crystallization on EC 1.1.1.290 - 4-phosphoerythronate dehydrogenase

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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour diffusion at 24C K using 0.7 M ammonium dihydrogen phosphate, 0.4 M ammonium tartrate, 0.1 M sodium citrate pH 5.6 and 10 mM cupric chloride
-
purified recombinant C-terminally His8-tagged and selenomethionine-labeled enzyme, subunit A is bound with NAD+ and a phosphate ion, while subunit B, with a more open active site cleft, is bound with NAD+ and L(+)-tartrate, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution, asymmetric subunit conformation and strucure comparison, overview