1.1.1.286: isocitrate-homoisocitrate dehydrogenase

This is an abbreviated version!
For detailed information about isocitrate-homoisocitrate dehydrogenase, go to the full flat file.

Reaction

Synonyms

3-carboxy-2-hydroxyadipate dehydrogenase, HICDH, homoisocitrate dehydrogenase, isocitrate-homoisocitrate dehydrogenase, More, Saci_2375, TK0280

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.286 isocitrate-homoisocitrate dehydrogenase

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Results	in table
13	AA Sequence
2	CAS Registry Number
5	Cloned(Commentary)
8	Cofactor
2	Crystallization (Commentary)
14	General Information
11	Inhibitors
36	kcat/KM [mM/s]
4	Ki Value [mM]
51	KM Value [mM]
9	Metals/Ions
3	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
12	Organism
15	Pathway
4	pH Optimum
1	pH Range
17	Protein Variants
4	Purification (Commentary)
4	Reaction
12	Reference
42	Substrates and Products (Substrate)
9	Subunits
20	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
33	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.286 - isocitrate-homoisocitrate dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant enzyme expressed in Escherichia coli, purification of inclusion bodies requires N-laurylsarcosine

Pyrococcus horikoshii

-

660907

recombinant enzyme from Escherichia coli by anion exchange chromatography, ammonium sufate fractionation, hydrophobic interaction chromatography, and gel filtration

Thermus thermophilus

Q72IW9

740000

recombinant enzyme from Escherichia coli strain BL21(DE3)Codon-Plus-RIL b anion exchange chromatography, ammonium sulfate fractionation, hydropobic interaction chromatography, ultrafiltration, and gel filtration

Sulfolobus acidocaldarius

Q4J6C9

738195

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration

Thermococcus kodakarensis

Q5JFV8

740021