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1.1.1.27: L-lactate dehydrogenase

This is an abbreviated version!
For detailed information about L-lactate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.27

Reaction

(S)-lactate
+
NAD+
=
pyruvate
 +
NADH
 +
H+

Synonyms

A4-LDH, AdhE, alpha-HBDH, alpha-hydroxybutyrate dehydrogenase, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, HBDH, heart LDH, Immunogenic protein p36, L(+)-nLDH, L-(+)-lactate dehydrogenase, L-lactate dehydrogenase, L-lactate dehydrogenase B, L-lactic acid dehydrogenase, L-lactic dehydrogenase, L-LDH, L-Ldh0845, L-nLDH, lactate dehydrogenase, lactate dehydrogenase 5, lactate dehydrogenase A, lactate dehydrogenase B, lactate dehydrogenase NAD-dependent, lactic acid dehydrogenase, lactic dehydrogenase, LctD, LDH, LDH-1, LDH-2, LDH-3, LDH-4, LDH-5, LDH-A, LDH-A4, LDH-m4, LDH1, LDH2, LdhA, LDHB, ldhL, LDHL1, LDHL2, LOX, LutACB, mL-LDH, mLDH, More, muscle LDH, NAD-dependent lactate dehydrogenase, NAD-lactate dehydrogenase, NGO0904, NGO0905, NGO0906, nitric oxideinducible l-lactate dehydrogenase, PfLDH, proteins, specific or class, anoxic stress response, p34, Sa-LDH-1, SMU.1115, SMU_1115, TeLdhL, Tsac_0416

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.27 L-lactate dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.27 - L-lactate dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.11
wild-type, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate
0.58
mutant R157L, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate
0.6
lactate oxidation, pH 8.0, 25°C, healthy breast tissue
0.65
wild-type, pH 7.3, 55°C
0.714
-
isoenzyme I
1.237
NAD+ reduction, pH 8.0, 25°C, healthy breast tissue
1.27
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate
1.282
NAD+ reduction, pH 8.0, 25°C, breast cancer tissue
1.37
NADH oxidation, pH 8.0, 25°C, healthy breast tissue
1.44
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C
1.747
pyruvate reduction, pH 8.0, 25°C, healthy breast tissue
1.87
-
isoenzyme II
10.4
-
pH 8.5, 25°C, substrate pyruvate
11.5
-
pyruvate decarboxylase 1/alcohol dehydrogenase 1 mutant strain AF297C
144.8
substrate pyruvate, pH 6, 25°C
15.5
wild-type, substrate benzoylformic acid, pH 5.5, 30°C
15.8
-
purified native anoxic enzyme, pH 7.2, 25°C, pyruvate reduction
16.48
substrate 3,4-dihydroxyphenylpyruvate, pH 6, 25°C
168.4
wild-type, substrate 2-oxobutanoate, pH 5.5, 30°C
17.4
wild-type, substrate 4-hydroxyphenylpyruvate, pH 5.5, 30°C
2.788
NADH oxidation, pH 8.0, 25°C, breast cancer tissue
203.3
-
purified native enzyme, substrate pyruvate
2119
-
recombinant enzyme
215
-
reduction of pyruvate
216
-
reduction of pyruvate
2323
purified recombinant enzyme, pH 6.5, 55°C, pyruvate reduction
25 - 40
wild-type, substrate pyruvate, pH 5.5, 30°C
253
reduction of pyruvate
29.6
-
pH 6.5, 25°C, substrate L-lactate
30
-
commercial preparation
351
purified recombinant enzyme, pH 11.5, 55°C, lactate oxidation
4.034
pyruvate reduction, pH 8.0, 25°C, breast cancer tissue
4071
mutant Q88R, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C
435.8
mutant I229A, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C
450
reduction of pyruvate
451.5
mutant Q88R, substrate phenylpyruvate, pH 5.5, 30°C
456
-
purified protein with pyruvate as substrate
458.5
-
-
5.1
-
single pyruvate decarboxylase 1 mutant strain T157
512.4
mutant I229A, substrate phenylpyruvate, pH 5.5, 30°C
53
Agama stellio stellio
-
-
66.7
mutant T235G, substrate phenylpyruvate, pH 5.5, 30°C
671.2
wild-type, substrate oxaloacetic acid, pH 5.5, 30°C
680.3
mutant T235G, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C
7.35
-
purified native aerobic control enzyme, pH 7.2, 25°C, pyruvate reduction
72.3
wild-type, substrate phenylpyruvate, pH 5.5, 30°C
798
wild-type, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C
80
-
isoenzyme X
80.27
Molinema dessetae
-
-
9.3 - 12.2
-
native enzyme in strain MG1363 at different growth phases and conditions, pH 7.0, 30°C
additional information