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1.1.1.263: 1,5-anhydro-D-fructose reductase

This is an abbreviated version!
For detailed information about 1,5-anhydro-D-fructose reductase, go to the full flat file.

Word Map on EC 1.1.1.263

Reaction

1,5-anhydro-D-glucitol
+
NADP+
=
1,5-Anhydro-D-fructose
+
NADPH
+
H+

Synonyms

1,4-AF-reductase, AF reductase, AFR, AKR1E1, More, NADP(H)-dependent 1,5-anhydro-D-fructose reductase, NADP-1,5-anhydro-D-fructose reductase, NADPH dependent 1,5-anhydro-D-fructose reductase, NADPH-dependent anhydrofructose reductase, NADPH-dependent monomeric reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.263 1,5-anhydro-D-fructose reductase

Purification

Purification on EC 1.1.1.263 - 1,5-anhydro-D-fructose reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration
-
Q-Sepharose HP column chromatography, Superdex 200 HR10 30 column chromatography and Red Sepharose CL-6B chromatography
-
recombinant enzyme 17.4fold from Escherichia coli strain BL21(DE3) by anion exchange and adsorption chromatography, and gel filtration
-
to homogeneity, chromatography techniques
-