1.1.1.214: 2-dehydropantolactone reductase (Si-specific)

This is an abbreviated version, for detailed information about 2-dehydropantolactone reductase (Si-specific), go to the full flat file.

Reaction

(R)-pantolactone
+
NADP+
=
2-dehydropantolactone
+
NADPH
+
H+

Synonyms

2-dehydropantoyl-lactone reductase (B-specific), 2-ketopantoyl lactone reductase, 2-oxopantoyl lactone reductase, conjugated polyketone reductase, conjugated polyketone reductase C2, CPR-C1, CPR-C2, ketopantoyl lactone reductase, NADPH-dependent conjugated polyketone reductase, NADPH-dependent ketopantoyl lactone reductase, nicotinamide adenine dinucleotide phosphate-dependent ketopantoyl lactone reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.214 2-dehydropantolactone reductase (Si-specific)

Engineering

Engineering on EC 1.1.1.214 - 2-dehydropantolactone reductase (Si-specific)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D58A
site-directed mutagenesis, the mutant shows 4.82% of wild-type activity
F299A
site-directed mutagenesis, the mutant shows 19.1% of wild-type activity
F300A
site-directed mutagenesis, the mutant shows 17.8% of wild-type activity
H125A
site-directed mutagenesis, the mutant shows 1.5% of wild-type activity
K264A
site-directed mutagenesis, the mutant shows 65.7% of wild-type activity
K28A
site-directed mutagenesis, the mutant shows 71.1% of wild-type activity
K30A
site-directed mutagenesis, the mutant shows 55.1% of wild-type activity
K88A
site-directed mutagenesis, inactive mutant
R267A
site-directed mutagenesis, the mutant shows 8.43% of wild-type activity
T27A
site-directed mutagenesis, the mutant shows 5.75% of wild-type activity
Y63A
site-directed mutagenesis, the mutant shows 0.17% of wild-type activity
D58A
-
site-directed mutagenesis, the mutant shows 4.82% of wild-type activity
-
H125A
-
site-directed mutagenesis, the mutant shows 1.5% of wild-type activity
-
K28A
-
site-directed mutagenesis, the mutant shows 71.1% of wild-type activity
-
K30A
-
site-directed mutagenesis, the mutant shows 55.1% of wild-type activity
-
R267A
-
site-directed mutagenesis, the mutant shows 8.43% of wild-type activity
-