1.1.1.159: 7alpha-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 7alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.159

1.1.1.159

chenodeoxycholic

cholic

testosteroni

bacteroides

thin-layer

3alpha-hydroxysteroid

synthesis

fragilis

7alpha-oh

glycochenodeoxycholic

nadp-dependent

ursodeoxycholic

perfringens

degradation

analysis

Reaction

3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oate

Synonyms

7-alpha-HSDH, 7-HSDH, 7alpha-HSD, 7alpha-HSDH, 7alpha-hydroxy steroid dehydrogenase, 7alpha-hydroxysteroid dehydrogenase, Bile acid-inducible protein, CA 7alpha-HSDH, Ca7alpha-HSDH, dehydrogenase, 7alpha-hydroxy steroid, J-1-1, NADP(H)-dependent 7alpha-HSDH, NADP(H)-dependent 7alpha-hydroxysteroid dehydrogenase, NADP+-dependent 7alpha-HSDH, nicotinamide adenine dinucleotide phosphate-dependent 7alpha-hydroxysteroid dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.159 7alpha-hydroxysteroid dehydrogenase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
9	Activating Compound
1020	AA Sequence
8	Application
1	CAS Registry Number
13	Cloned(Commentary)
40	Cofactor
4	Crystallization (Commentary)
12	General Information
4	General Stability
37	Inhibitors
13	kcat/KM [mM/s]
61	KM Value [mM]
10	Metals/Ions
16	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
57	Organism
3	Pathway
26	PDB ID
16	pH Optimum
3	pH Range
4	pH Stability
1	pI Value
33	Protein Variants
17	Purification (Commentary)
4	Reaction
3	Reaction Type
45	Reference
2	Source Tissue
31	Specific Activity [micromol/min/mg]
4	Storage Stability
133	Substrates and Products (Substrate)
15	Subunits
38	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
15	Temperature Stability [°C]
24	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Cloned on EC 1.1.1.159 - 7alpha-hydroxysteroid dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Cloned (commentary) Search

CA 7alpha-HSDH, recombinant overexpression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Clostridium sardiniense

G9FRD7

740787

expressed in Escherichia coli

3 entries

expressed in Escherichia coli BL21(DE3) cells

Clostridium sardiniense

G9FRD7

760413, 760712

expression in Escherichia coli DH5alpha-MCR, DNA and amino acid sequence analysis

Paeniclostridium sordellii

286084

gene 7alpha-HSD, located 11.9 kb upstream of the hsdA gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain HB101, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS

Comamonas testosteroni

S5U393

740971

gene from strain HB101 cloned in plasmid pSD1 and expressed in Escherichia coli strain DH1

Escherichia coli

286085

overexpression in Escherichia coli

Bacteroides fragilis

670926

overexpression in Escherichia coli DH5alpha, amino acid sequence analysis and comparison with other dehydrogenases

Eubacterium sp.

286082

recombinant expression of wild-type and truncation mutant enzymes in Escherichia coli

Clostridium sardiniense

G9FRD7

741371

site directed mutagenesis

Escherichia coli

286083