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1.1.1.12: L-arabinitol 4-dehydrogenase

This is an abbreviated version!
For detailed information about L-arabinitol 4-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.12

Reaction

L-arabinitol
+
NAD+
=
L-xylulose
+
NADH
+
H+

Synonyms

ADH, dehydrogenase, L-arabinitol, HjLAD, L-arabinitol dehydrogenase, L-arabitol dehydrogenase, LAD, Lad1, LADA, More, NAD(P)+-dependent arabitol dehydrogenase, pentitol-DPN dehydrogenase, XYL2

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.12 L-arabinitol 4-dehydrogenase

Cloned

Cloned on EC 1.1.1.12 - L-arabinitol 4-dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells
expression in Escherichia coli
gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene lad1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ladA, DNA and amino acid sequence determination and analysis
H6WCP4, JQ079782
gene ladA, DNA and amino acid sequence determination and analysis, construction of strain 3M-43/pyrA, a pyrA mutant of strain 3M-43, in which an intact copy of ladA is inserted under control of the Aspergillus nidulans gpdA promoter. Complementation of the 3M-43 pyrA strain with pGpd:ladA
H6WCP4, JQ079782
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)