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0.25
(+/-)-3-methylcyclohexanone
-
-
9.1
(2E)-but-2-en-1-ol
pH 8.0, 55°C
3.3
(2E)-but-2-enal
pH 6.5, 65°C
0.41 - 37.4
(R)-2-butanol
0.22 - 31.7
(R)-2-pentanol
0.02
(R)-2-phenylpropanol
-
-
1.28 - 1.3
(R)-alpha-tetralol
4.2
(S)-(+)-1-indanol
-
65°C
4.2
(S)-(+)-alpha-tetraol
-
65°C
18.1
(S)-(-)-1-phenylethanol
-
65°C
18.1
(S)-1-phenylethanol
-
pH 10.5, 65°C
0.012 - 120
(S)-2-butanol
0.07 - 8.8
(S)-2-pentanol
0.157
(S)-2-phenylpropanol
-
-
1.05 - 4.2
(S)-alpha-tetralol
8.47
(S)-N-benzyl-3-pyrrolidinol
-
pH 8.0
1.65 - 15.84
1,2-propanediol
23.64 - 26.7
1,3-Propanediol
0.36
1,4-Butanediol
pH 10, 60°C
0.000036 - 0.0038
1-formyl-6-methylpyrene
0.000035 - 0.00149
1-formyl-8-methylpyrene
0.0005 - 0.012
1-formylpyrene
0.00028 - 0.0064
1-hydroxymethyl-6-methylpyrene
0.00031 - 0.059
1-hydroxymethyl-8-methylpyrene
0.00059 - 0.076
1-hydroxymethylpyrene
5.9
1-phenyl-1,2-propandione
-
pH 10.5, 65°C
5 - 12.1
1-phenyl-1,2-propanedione
0.025 - 244
1-phenylethanol
0.2
11-cis-retinal
-
reduction with NADH
0.018 - 0.28
11-cis-retinol
0.013 - 2.3
12-hydroxydodecanoate
0.056
12-Hydroxydodecanoic acid
-
-
0.086 - 0.1
12-hydroxylauric acid methyl ester
0.066
12-oxolauric acid methyl ester
wild-type, pH 8.0, 60°C
0.11 - 0.137
13-cis-retinal
0.257
13-cis-retinol
-
oxidation with NAD+
0.06
16-hydroxyhexadecanoate
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
11.9
2',3',4',5',6'-pentafluoroacetophenone
65°C, pH 5.0
6.3 - 23.5
2,2,2-trifluoroacetophenone
0.11
2,2-dichloroacetophenone
65°C, pH 5.0
0.44 - 1100
2,3-Butanediol
0.147
2-decanone
pH 8.0, 60°C
13
2-dehydro-3-deoxy-D-gluconate
at pH 7.9 and 25°C
310
2-deoxy-D-ribose
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.25
2-ethoxyethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.0021 - 0.009
2-formylpyrene
1.16
2-Heptanone
pH 8.0, 60°C
5.01
2-Hexanone
pH 8.0, 60°C
0.00048 - 0.106
2-hydroxymethylpyrene
0.021
2-Methylcyclohexanol
-
-
2.7
2-methylpropan-1-ol
-
-
0.215
2-nonanone
pH 8.0, 60°C
0.286
2-Octanone
pH 8.0, 60°C
0.752
2-Pentanol
pH 8.0, 60°C
0.864
2-phenylpropionaldehyde
-
-
0.025 - 0.026
3,4-dihydro-retinal
0.024 - 0.028
3,4-dihydro-retinol
0.14 - 0.9
3-bromobenzyl alcohol
0.14
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.9 - 13
3-hydroxy-2-butanone
4.4
3-Methoxybenzaldehyde
-
65°C
1.2 - 3.3
3-Methoxybenzyl alcohol
1.2
3-methoxybenzylalcohol
7.5
3-methylbutan-2-one
-
-
9.9
3-Methylcyclohexanol
65°C, pH 8.0
66
3-methylcyclohexanone
65°C, pH 5.5
0.031
3-oxo-5beta-androstan-17beta-ol
-
-
0.032
3-Phenyl-1-propanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.033
3-phenylpropanol
-
-
0.128 - 0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
0.149 - 0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
0.35
3beta,7alpha-12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 4
0.032 - 0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
3.8 - 34.9
4-amino-1-butanol
0.12 - 0.4
4-bromobenzyl alcohol
0.12
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.28
4-carboxybenzaldehyde
0.14 - 1.11
4-chloroacetophenone
0.00048 - 0.0038
4-formylpyrene
0.004 - 0.015
4-hydroxy-retinol
0.00037 - 0.04
4-hydroxymethylpyrene
0.024 - 4.4
4-methoxybenzaldehyde
0.07 - 61
4-methoxybenzyl alcohol
1.3
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.131
4-methoxyphenylacetone
pH 8.0, 60°C
6.7 - 53.8
4-Methyl-1-pentanol
0.81
4-methylpentan-1-ol
-
-
0.017 - 0.027
4-oxo-retinal
26.9
5-amino-1-pentanol
pH 9.0, 25°C, recombinant enzyme
9.45
5-hydroxymethylfurfural
-
mutant S110P/Y295C, pH 6.7, 30°C
0.0036 - 0.04
5alpha-androstan-17beta-ol-3-one
0.011 - 0.046
5beta-androstan-17beta-ol-3-one
0.123 - 0.164
5beta-androstan-3beta-ol-17 one
0.003 - 0.058
5beta-androstan-3beta-ol-17-one
0.008 - 0.25
5beta-cholanic acid-3-one
0.047
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
-
-
0.0036
5beta-pregnan-3,20-dione
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.027
5beta-Pregnan-3beta-ol-20-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
6.71
6-amino-1-hexanol
pH 9.0, 25°C, recombinant enzyme
0.16 - 0.242
9-cis-retinal
0.011 - 0.3
9-cis-retinol
0.0086 - 121
acetaldehyde
0.011 - 0.34
all-trans-retinal
0.009 - 0.059
all-trans-retinol
0.39
allyl alcohol
-
pH 7.3, 37°C
5.3
alpha-tetralol
-
65°C
0.0025 - 0.09
Anisaldehyde
0.007 - 49
benzyl alcohol
0.0145 - 0.8
benzylalcohol
86.8
Butan-2,3-diol
pH 8.8, 70°C
0.06 - 0.27
Butyraldehyde
0.14 - 0.25
capronaldehyde
0.03
cinnamaldehyde
-
pH 7.7, 60°C
0.11 - 4.58
cinnamyl alcohol
1
cis-4-methylcyclohexanol
-
-
4.2
cis-decahydro-1-naphthol
65°C, pH 8.0
0.71
crotyl alcohol
-
pH 7.3, 37°C
0.008 - 1900
Cyclohexanol
1.39 - 14.1
cyclohexanone
16
D-allonate
at pH 7.9 and 25°C
24
D-altronate
at pH 7.9 and 25°C
220
D-arabitol
at pH 7.9 and 25°C
12
D-gluconate
at pH 7.9 and 25°C
14
D-mannonate
at pH 7.9 and 25°C
16
D-ribonate
at pH 7.9 and 25°C
350
D-sorbitol
at pH 7.9 and 25°C
6
D-talonate
at pH 7.9 and 25°C
24
D-xylonate
at pH 7.9 and 25°C
16.6
ethyl 3-methyl-2-oxobutyrate
65°C, pH 5.0
1 - 5.3
ethyl benzoylformate
1
ethyl oxo(phenyl)acetate
-
pH 10.5, 65°C
5.3
ethyl pyruvate
-
pH 6.5, 50°C, recombinant enzyme
0.67
Glutaraldehyde
pH 6, 60°C
16.11 - 88.32
glycolaldehyde
0.81
isoborneol
65°C, pH 10.5
0.26 - 0.76
Isobutyraldehyde
197
Isopentanol
at pH 7.0 and 25°C
1.4
m-nitrobenzaldehyde
-
isoenzyme ADH-1, pH 7.5
6.5
methyl benzoylformate
65°C, pH 5.5
2.8
methyl o-chlorobenzoylformate
2.7
methyl oxo(phenyl)acetate
-
pH 10.5, 65°C
2.7
methylbenzoylformate
-
65°C
0.13 - 0.58
N-benzyl-3-pyrrolidinone
10.3
n-Pentanal
-
33°C, pH 8
0.011
octan-1-ol
-
enzyme form ADH-3
2.9 - 5.9
p-Methoxybenzaldehyde
6.9 - 22
p-methoxybenzyl alcohol
13.6
phenyl trifluoromethyl ketone
-
25°C, pH 7.0
0.19 - 0.91
phenylacetaldehyde
24.45 - 37.55
phenylglyoxylic acid
2.01
propanone
-
33°C, pH 7
3.4
R-(+)-1-phenylethanol
-
-
1.2
R-(+)-cis-verbenol
-
-
1.5
R-(+)-trans-bicyclo(2.2.1)-heptanol
-
-
3.9
S-(-)-1-phenylethanol
-
-
0.95
S-(-)-2-methylbutan-1-ol
-
-
3.8
S-(-)-cis-bicyclo(2.2.1)-heptanol
-
-
0.85
S-(-)-trans-bicyclo(2.2.1)-heptanol
-
-
568.84
tert-butanol
pH 7.5, 23°C
0.694
tert-butyl acetoacetate
pH 8.0, 60°C
8.5
tetralin-1-ol
65°C, pH 10.5
1.1
trans-4-methylcyclohexanol
-
-
0.009 - 0.23
trans-cinnamaldehyde
0.2
tryptophol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.034 - 11
Vanillyl alcohol
additional information
additional information
-
2.4
(R)-1-indanol
pH 8.0, 65°C
2.4
(R)-1-indanol
65°C, pH 8.0
0.41
(R)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
37.4
(R)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.22
(R)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.7
(R)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.28
(R)-alpha-tetralol
pH 8.0, 65°C
1.3
(R)-alpha-tetralol
65°C, pH 8.0
4.2
(S)-1-indanol
-
pH 10.5, 65°C
6.4
(S)-1-indanol
pH 8.0, 65°C
6.4
(S)-1-indanol
65°C, pH 8.0
0.012
(S)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29.8
(S)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
(S)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
8.8
(S)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.05
(S)-alpha-tetralol
pH 8.0, 65°C
1.05
(S)-alpha-tetralol
65°C, pH 8.0
4.2
(S)-alpha-tetralol
-
pH 10.5, 65°C
1.65
1,2-propanediol
-
-
11.5
1,2-propanediol
-
pH 8.0, 60°C, recombinant enzyme
15.84
1,2-propanediol
-
-
23.64
1,3-Propanediol
pH 7.5, 23°C
26.7
1,3-Propanediol
-
pH 9.0, 60°C, recombinant enzyme
0.08
1-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
1-butanol
-
isoenzyme ADH-3, pH 10.0
0.596
1-butanol
pH 8.0, 60°C
1.6
1-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.45
1-butanol
pH 7.5, 23°C
17
1-butanol
-
isoenzyme ADH-1, pH 10.0
230
1-butanol
-
isoenzyme ADH-1, pH 7.5
0.000036
1-formyl-6-methylpyrene
isozyme ADH4, at 21-23°C
0.00032
1-formyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.0009
1-formyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0038
1-formyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.000035
1-formyl-8-methylpyrene
isozyme ADH4, at 21-23°C
0.00024
1-formyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.00131
1-formyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00149
1-formyl-8-methylpyrene
isozyme ADH3, at 21-23°C
0.0005
1-formylpyrene
isozyme ADH1C, at 21-23°C
0.00055
1-formylpyrene
isozyme ADH3, at 21-23°C
0.00092
1-formylpyrene
isozyme ADH4, at 21-23°C
0.012
1-formylpyrene
isozyme ADH2, at 21-23°C
0.038
1-Heptanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.21
1-Heptanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.67
1-Heptanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.035
1-Hexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
1-Hexanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.147
1-Hexanol
pH 8.0, 60°C
0.88
1-Hexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.00028
1-hydroxymethyl-6-methylpyrene
isozyme ADH4, at 21-23°C
0.00057
1-hydroxymethyl-6-methylpyrene
isozyme ADH3, at 21-23°C
0.00115
1-hydroxymethyl-6-methylpyrene
isozyme ADH1C, at 21-23°C
0.0064
1-hydroxymethyl-6-methylpyrene
isozyme ADH2, at 21-23°C
0.00031
1-hydroxymethyl-8-methylpyrene
isozyme ADH3, at 21-23°C
0.0016
1-hydroxymethyl-8-methylpyrene
isozyme ADH4, at 21-23°C
0.0064
1-hydroxymethyl-8-methylpyrene
isozyme ADH2, at 21-23°C
0.059
1-hydroxymethyl-8-methylpyrene
isozyme ADH1C, at 21-23°C
0.00059
1-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
0.00075
1-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0283
1-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.076
1-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
5.1
1-Indanol
-
65°C
8.7
1-Indanol
65°C, pH 10.5
27.6
1-Indanone
-
65°C
27.6
1-Indanone
-
pH 10.5, 65°C
0.025
1-Octanol
-
isoenzyme ADH-3, pH 10.0
0.1
1-Octanol
-
isoenzyme ADH-2
0.39
1-Octanol
isozyme ADH3, at 21-23°C
0.5
1-Octanol
-
isoenzyme ADH-1, pH 7.5
0.51
1-Octanol
-
isoenzyme ADH-2, pH 10.0
0.027
1-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.08
1-Pentanol
-
isoenzyme ADH-3, pH 10.0
0.26
1-Pentanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.396
1-Pentanol
pH 8.0, 60°C
1.9
1-Pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.1
1-Pentanol
-
isoenzyme ADH-1, pH 10.0
78
1-Pentanol
-
isoenzyme ADH-2, pH 10.0
5
1-phenyl-1,2-propanedione
65°C, pH 5.0
5.9
1-phenyl-1,2-propanedione
-
65°C
12.1
1-phenyl-1,2-propanedione
65°C, pH 5.5
0.025
1-phenylethanol
-
-
244
1-phenylethanol
pH 9.0, 70°C
0.24
1-propanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.33
1-propanol
-
native enzyme, at 55°C
0.6
1-propanol
-
wild type enzyme, at 65°C
1.03
1-propanol
pH 8.0, 60°C
1.7
1-propanol
-
mutant enzyme N249Y, at 65°C
2.4
1-propanol
-
carboxymethylated enzyme, at 55°C
6.5
1-propanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
39.8
1-propanol
pH 7.5, 23°C
0.018
11-cis-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.035
11-cis-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.28
11-cis-retinol
-
oxidation with NAD+
0.013
12-hydroxydodecanoate
-
isoenzyme ADH-3, pH 10.0
0.02
12-hydroxydodecanoate
-
isoenzyme BB-ADH
0.04
12-hydroxydodecanoate
-
enzyme form ADH-3
0.047
12-hydroxydodecanoate
-
-
0.048
12-hydroxydodecanoate
-
-
0.05
12-hydroxydodecanoate
-
isoenzyme BB-ADH and isoenzyme ADH-TT
0.06
12-hydroxydodecanoate
-
isoenzyme AA-ADH
0.1
12-hydroxydodecanoate
-
-
0.1
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
0.23
12-hydroxydodecanoate
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
1.4
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
2.3
12-hydroxydodecanoate
-
isoenzyme TT-ADH
0.086
12-hydroxylauric acid methyl ester
wild-type, pH 8.0, 60°C
0.1
12-hydroxylauric acid methyl ester
mutant C257L, pH 8.0, 60°C
0.11
13-cis-retinal
-
oxidation with NAD+
0.137
13-cis-retinal
-
reduction with NADH
6.3
2,2,2-trifluoroacetophenone
65°C, pH 5.0
11.2
2,2,2-trifluoroacetophenone
-
65°C
11.2
2,2,2-trifluoroacetophenone
-
pH 10.5, 65°C
23.5
2,2,2-trifluoroacetophenone
65°C, pH 5.5
0.44
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+
0.91
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+
1.3
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, wild-type enzyme
1.5
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+
5.5
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+
9.6
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+
12
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+
17
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+
29
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, wild-type enzyme
61.3
2,3-Butanediol
pH 9.0, 70°C
76
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+
90
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+
100
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+
110
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+
200
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, mutant enzyme K249G/H255R
240
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+
260
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+
690
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, mutant enzyme K249G/H255R
1100
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+
1.05
2-butanol
pH 8.0, 60°C
11.9
2-butanol
-
33°C, pH 8
23.82
2-butanol
pH 7.5, 23°C
0.35
2-Buten-1-ol
-
isoenzyme ADH-3, pH 10.0
2.5
2-Buten-1-ol
-
isoenzyme ADH-1, pH 10.0
60
2-Buten-1-ol
-
isoenzyme ADH-2, pH 10.0
0.0021
2-formylpyrene
isozyme ADH1C, at 21-23°C
0.0029
2-formylpyrene
isozyme ADH2, at 21-23°C
0.0069
2-formylpyrene
isozyme ADH4, at 21-23°C
0.009
2-formylpyrene
isozyme ADH3, at 21-23°C
0.00048
2-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0044
2-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.033
2-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.106
2-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
5.15
2-Pentanone
pH 8.0, 60°C
0.6
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.6
2-propanol
-
native enzyme, at 55°C
2.44
2-propanol
pH 8.0, 60°C
19.6
2-propanol
-
33°C, pH 8
53
2-propanol
-
carboxymethylated enzyme, at 55°C
57.5
2-propanol
-
25°C, pH 7.0
57.8
2-propanol
-
pH 7.7, 60°C
268
2-propanol
-
wilde-type enzyme ADh1
560
2-propanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.025
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.026
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.024
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.028
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.14
3-bromobenzyl alcohol
-
native enzyme, at 55°C
0.9
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.9
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, wild-type enzyme
5
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, mutant enzyme K249G/H255R
6.7
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, wild-type enzyme
13
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, mutant enzyme K249G/H255R
1.2
3-Methoxybenzyl alcohol
-
native enzyme, at 55°C
3.3
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
3-methoxybenzylalcohol
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.27
3-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.128
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 2
0.16
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 3
0.248
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
0.31
3beta,12alpha-dihydroxy-5beta-cholanoate
-
isoenzyme I
0.149
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme I
0.16
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 2
0.182
3beta,7alpha,12alpha-trihydroxy-5beta-cholanoate
-
isoenzyme 3
0.032
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 3
0.064
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 2
0.066
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 1
0.071
3beta-7alpha-dihydroxy-5beta-cholanoate
-
isoenzyme 4
3.8
4-amino-1-butanol
pH 11.0, 25°C, recombinant enzyme
17.8
4-amino-1-butanol
pH 9.0, 25°C, recombinant enzyme
34.9
4-amino-1-butanol
pH 7.0, 25°C, recombinant enzyme
0.12
4-bromobenzyl alcohol
-
native enzyme, at 55°C
0.4
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.28
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.28
4-carboxybenzaldehyde
-
native enzyme, at 55°C
0.14
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.53
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
1.11
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.00048
4-formylpyrene
isozyme ADH4, at 21-23°C
0.001
4-formylpyrene
isozyme ADH1C, at 21-23°C
0.0021
4-formylpyrene
isozyme ADH3, at 21-23°C
0.0038
4-formylpyrene
isozyme ADH2, at 21-23°C
0.004
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.011
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.015
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.00037
4-hydroxymethylpyrene
isozyme ADH3, at 21-23°C
0.001
4-hydroxymethylpyrene
isozyme ADH1C, at 21-23°C
0.0029
4-hydroxymethylpyrene
isozyme ADH4, at 21-23°C
0.04
4-hydroxymethylpyrene
isozyme ADH2, at 21-23°C
0.024
4-methoxybenzaldehyde
-
wild type enzyme, at 65°C
0.14
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.14
4-methoxybenzaldehyde
-
native enzyme, at 55°C
0.86
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
4.4
4-methoxybenzaldehyde
-
pH 6.0, 65°C
0.07
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.12
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
1.13
4-methoxybenzyl alcohol
pH 8.0, 60°C
1.24
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.3
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
1.3
4-methoxybenzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
61
4-methoxybenzyl alcohol
-
65°C
61
4-methoxybenzyl alcohol
-
pH 10.5, 65°C
6.7
4-Methyl-1-pentanol
-
mutant enzyme W54L
53.8
4-Methyl-1-pentanol
-
wilde-type enzyme ADh1
0.017
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.025
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.027
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.0036
5alpha-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.021
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.025
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 2
0.027
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 4
0.04
5alpha-androstan-17beta-ol-3-one
-
isoenzyme 1
0.011
5beta-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, recombinant enzyme
0.046
5beta-androstan-17beta-ol-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.123
5beta-androstan-3beta-ol-17 one
-
isoenzyme 4
0.125
5beta-androstan-3beta-ol-17 one
-
isoenzyme 2
0.14
5beta-androstan-3beta-ol-17 one
-
isoenzyme 3
0.164
5beta-androstan-3beta-ol-17 one
-
isoenzyme 1
0.003
5beta-androstan-3beta-ol-17-one
-
pH 7.3, 37°C, recombinant enzyme
0.058
5beta-androstan-3beta-ol-17-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.008
5beta-cholanic acid-3-one
-
pH 7.3, 37°C, recombinant enzyme
0.25
5beta-cholanic acid-3-one
-
pH 7.3, 37°C, ADH1C*2 (gamma2gamma2)
0.16
9-cis-retinal
-
oxidation with NAD+
0.242
9-cis-retinal
-
reduction with NADH
0.011
9-cis-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.023
9-cis-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.3
9-cis-retinol
-
oxidation with NAD+
0.0086
acetaldehyde
Sporotrichum pulverulentum
-
-
0.013
acetaldehyde
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.059
acetaldehyde
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.085
acetaldehyde
-
isoenzyme beta1,beta1
0.085
acetaldehyde
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
0.086
acetaldehyde
-
enzyme form ADH I
0.1
acetaldehyde
-
pyrazole-sensitive enzyme, pH 7.0
0.217
acetaldehyde
-
isoenzyme 3
0.22
acetaldehyde
-
wild-type, pH 6.7, 30°C
0.23
acetaldehyde
-
isoenzyme 2
0.24
acetaldehyde
-
isoenzyme gamma2,gamma2
0.24
acetaldehyde
-
isoenzymes beta2,beta2
0.24
acetaldehyde
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
0.276
acetaldehyde
-
isoenzyme 4
0.3
acetaldehyde
-
isozyme ADH I, pH 5.5, 25°C
0.31
acetaldehyde
-
isoenzyme 1
0.33
acetaldehyde
-
isoenzyme gamma1,gamma1
0.34
acetaldehyde
isozyme ADH1C, at 21-23°C
0.364
acetaldehyde
wild-type, pH 8.0, 60°C
0.67
acetaldehyde
pH 6.0, 22°C, recombinant enzyme
0.74
acetaldehyde
pH 7.0, 30°C, with NADH
0.83
acetaldehyde
-
mutant S110P/Y295C, pH 6.7, 30°C
0.92
acetaldehyde
-
33°C, pH 7
1.2
acetaldehyde
pH 7.0, temperature not specified in the publication
1.25
acetaldehyde
-
isoenzyme II
1.3
acetaldehyde
-
enzyme form ADH II
1.45
acetaldehyde
-
isoenzyme I
1.8
acetaldehyde
Megalodesulfovibrio gigas
-
-
1.9
acetaldehyde
-
pH 8.5
2.6
acetaldehyde
-
pH 8.0, 60°C, recombinant enzyme
3.4
acetaldehyde
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
4
acetaldehyde
pH 7.0, temperature not specified in the publication
4.3
acetaldehyde
-
isoenzyme alpha,alpha
4.71
acetaldehyde
pH 8, 60°C
7.43
acetaldehyde
-
pH 7.0, 30°C
11.5
acetaldehyde
-
pyrazole-insensitive enzyme, pH 7.0
12.7
acetaldehyde
isozyme ADH4, at 21-23°C
24
acetaldehyde
-
isozyme ADH II, pH 6.0, 25°C
26
acetaldehyde
isozyme ADH2, at 21-23°C
28
acetaldehyde
pH 6.0, temperature not specified in the publication
34
acetaldehyde
-
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
70
acetaldehyde
-
mutant enzyme ADH1-1S1108
85
acetaldehyde
-
wild-type enzyme ADH1-1S
121
acetaldehyde
-
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
6.5
acetoin
reduction reaction, pH 6.1, 70°C
30.2
acetoin
pH 6.0, 70°C
6.6
acetone
-
-
0.2
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.4
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.66
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.3
acycloNAD+
-
substrate butan-1-ol, pH 8.0, 25°C
0.33
acycloNAD+
-
substrate hexan-1-ol, pH 8.0, 25°C
0.33
acycloNAD+
-
substrate propan-1-ol, pH 8.0, 25°C
0.36
acycloNAD+
-
substrate ethanol, pH 8.0, 25°C
0.61
acycloNAD+
-
substrate benzyl alcohol, pH 8.0, 25°C
0.011
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.012
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
0.034
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.26
all-trans-retinal
-
oxidation with NAD+
0.34
all-trans-retinal
-
reduction with NADH
0.009
all-trans-retinol
-
-
0.02
all-trans-retinol
-
isoenzyme TT-ADH
0.023
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.03
all-trans-retinol
-
isoenzyme BB-ADH
0.03
all-trans-retinol
-
isozyme ADH1B1, pH 7.5, 25°C
0.03
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.033
all-trans-retinol
-
isozyme ADH1B2, pH 7.5, 25°C
0.033
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.05
all-trans-retinol
-
isoenzyme AA-ADH
0.059
all-trans-retinol
-
oxidation with NAD+
5.8
alpha-tetralone
-
65°C
5.8
alpha-tetralone
-
pH 10.5, 65°C
0.0025
Anisaldehyde
-
-
0.03
benzaldehyde
-
wild type enzyme, at 65°C
0.03
benzaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.07
benzaldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.2
benzaldehyde
-
carboxymethylated enzyme, at 55°C
0.26
benzaldehyde
-
mutant enzyme N249Y, at 65°C
0.28
benzaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.3
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.3
benzaldehyde
-
native enzyme, at 55°C
0.333
benzaldehyde
pH 8.0, 60°C
0.4
benzaldehyde
pH 6.5, 65°C
2 - 3
benzaldehyde
in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
14
benzaldehyde
-
isozyme ADH2, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
33
benzaldehyde
-
isozyme ADH1, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.007
benzyl alcohol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.013
benzyl alcohol
-
enzyme form ADH-2
0.05
benzyl alcohol
-
isoenzyme ADH-3, pH 10.0
0.12
benzyl alcohol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.26
benzyl alcohol
-
wild type enzyme, at 65°C
0.26
benzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.8
benzyl alcohol
-
native enzyme, at 55°C
0.8
benzyl alcohol
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
1.4
benzyl alcohol
-
isoenzyme ADH-1, pH 10.0
1.5
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
1.5
benzyl alcohol
mutant W87A, pH 7.0, 30°C
2
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
3.1
benzyl alcohol
mutant W49F/W167Y, 30°C, pH not specified in the publication
3.8
benzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.2
benzyl alcohol
mutant V260A, 30°C, pH not specified in the publication
5.4
benzyl alcohol
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
5.43
benzyl alcohol
pH 8.0, 60°C
6
benzyl alcohol
mutant W49F/W87F, 30°C, pH not specified in the publication
6.8
benzyl alcohol
wild-type, 30°C, pH not specified in the publication
6.9
benzyl alcohol
wild-type, pH 7.0, 30°C
7.2
benzyl alcohol
mutant Y25A, pH 7.0, 30°C
8.2
benzyl alcohol
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
10.1
benzyl alcohol
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
10.9
benzyl alcohol
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
12.9
benzyl alcohol
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
14
benzyl alcohol
pH 8.0, 55°C
16
benzyl alcohol
mutant A25Y, pH 7.0, 30°C
16.5
benzyl alcohol
mutant Y25A, 30°C, pH not specified in the publication
24
benzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
29.4
benzyl alcohol
wild-type, pH 7.0, 30°C
34
benzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
49
benzyl alcohol
-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.0145
benzylalcohol
in 50 mM glycine-NaOH at pH 10.0
0.44
benzylalcohol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.8
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.24
beta-NAD+
-
in presence of 0.5 mM SDS
0.243
beta-NAD+
-
in presence of 0.5 mM SDS
0.35
beta-NAD+
-
in presence of 1 mM SDS
0.71
beta-NAD+
-
in presence of 3 mM SDS
1.23
beta-NAD+
-
in presence of 50 mM SDS
2.29
beta-NAD+
-
in presence of 15 mM SDS
0.055
butan-1-ol
-
-
2
butan-1-ol
-
enzyme form ADH-3
5.65
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
0.35
butan-2-ol
-
-
93.3
butan-2-ol
-
wilde-type enzyme ADh1
0.48
Butanal
cosubstrate NADH, pH 7.0, 30°C
3
Butanal
-
isoenzyme ADH-1, pH 7.5
4.24
Butanal
-
33°C, pH 7
71.98
Butanal
-
pH 7.0, 30°C
0.012
butanol
-
pyrazole-sensitive enzyme, pH 7.5
0.02
butanol
-
pyrazole-sinensitive enzyme, pH 10.0
0.06
butanol
-
pyrazole-insensitive enzyme, pH 7.5
0.09
butanol
-
pyrazole-sensitive enzyme, pH 10.0
29.4
butanol
-
mutant enzyme W54L
53.9
butanol
-
wilde-type enzyme ADh1
0.06
Butyraldehyde
-
-
0.07
Butyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.108
Butyraldehyde
wild-type, pH 8.0, 60°C
0.13
Butyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.27
Butyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.14
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.22
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.25
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.11
cinnamyl alcohol
-
pH 7.7, 60°C
1.5
cinnamyl alcohol
-
mutant enzyme W54L
4.58
cinnamyl alcohol
-
wilde-type enzyme ADh1
5.1
cycloheptanol
65°C, pH 10.5
9.3
cycloheptanol
pH 8.0, 65°C
9.3
cycloheptanol
65°C, pH 8.0
0.008
Cyclohexanol
-
isoenzyme alpha,gamma1
0.03
Cyclohexanol
-
wild type enzyme, at 65°C
0.03
Cyclohexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.04
Cyclohexanol
-
isoenzyme BB-ADH
0.045
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.045
Cyclohexanol
-
native enzyme, at 55°C
0.23
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
0.703
Cyclohexanol
pH 8.0, 60°C
0.76
Cyclohexanol
-
isoenzyme AA-ADH
1.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
2.09
Cyclohexanol
in 200 mM bicine, pH 9.0, at 60°C
2.2
Cyclohexanol
-
isoenzyme ADH-3, pH 10.0
4.5
Cyclohexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
5.2
Cyclohexanol
-
enzyme form ADH-3
23
Cyclohexanol
-
isoenzyme beta1,beta1
210
Cyclohexanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
220
Cyclohexanol
-
isoenzyme ADH-1, pH 10.0
320
Cyclohexanol
-
isoenzyme TT-ADH
1900
Cyclohexanol
-
isoenzyme ADH-2, pH 10.0
1.39
cyclohexanone
pH 8.0, 60°C
3.68
cyclohexanone
in 200 mM bicine, pH 6.0, at 60°C
0.0172
ethanol
in 50 mM glycine-NaOH at pH 10.0
0.022
ethanol
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.022
ethanol
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
0.049
ethanol
-
isoenzyme beta1,beta1
0.147
ethanol
20°C, pH 9.0
0.15
ethanol
Megalodesulfovibrio gigas
-
-
0.166
ethanol
recombinant His-tagged wild-type enzyme, pH 7.5, temperature not specified in the publication
0.168
ethanol
30°C, pH 9.0
0.18
ethanol
-
pyrazole-sensitive enzyme, pH 7.5
0.19
ethanol
10°C, pH 9.0
0.248
ethanol
40°C, pH 9.0
0.251
ethanol
recombinant His-tagged mutant C243S, pH 7.5, temperature not specified in the publication
0.34
ethanol
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.43
ethanol
50°C, pH 9.0
0.45
ethanol
pH 9.0, 22°C, recombinant enzyme
0.48
ethanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.5
ethanol
-
isoenzyme AA-ADH
0.63
ethanol
-
isoenzyme gamma2,gamma2
0.7
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
ethanol
-
native enzyme, at 55°C
0.76
ethanol
-
isoenzyme 2
0.76
ethanol
-
isoenzyme BB-ADH
0.77
ethanol
isozyme ADH1C, at 21-23°C
0.8
ethanol
-
pyrazole-sensitive enzyme, pH 10.0
0.83
ethanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
0.84
ethanol
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
0.91
ethanol
wild-type, pH 8.0, 60°C
0.94
ethanol
-
isoenzyme beta2,beta2
1
ethanol
-
isoenzyme gamma1,gamma1
1.04
ethanol
60°C, pH 9.0
1.12
ethanol
-
in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C
1.33
ethanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
1.37
ethanol
-
isoenzyme 1
1.4
ethanol
-
isoenzyme ADH-3, pH 10.0 and pH 7.5
1.41
ethanol
-
isoenzyme 4
1.5
ethanol
-
isoenzyme alpha,alpha
1.5
ethanol
mutant C257L, pH 8.0, 60°C
1.6
ethanol
-
isoenzyme beta1,beta1
1.7
ethanol
-
isoenzyme III
1.7
ethanol
-
isoenzyme alpha,gamma1
1.7
ethanol
-
isoenzyme gamma2,gamma2
1.87
ethanol
-
isoenzyme 3
2.1
ethanol
-
in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C
2.3
ethanol
-
isozyme ADH I, pH 8.5, 25°C
2.46
ethanol
-
pH 7.3, 37°C
2.5
ethanol
Sporotrichum pulverulentum
-
-
2.95
ethanol
pH 7.5, 23°C
3.1
ethanol
at pH 9.0 in bis-Tris propane buffer
3.2
ethanol
-
isoenzyme gamma1,gamma1
3.2
ethanol
at pH 7.0 and 25°C
3.5
ethanol
-
isoenzyme III from leaf
3.6
ethanol
isozyme ADH4, at 21-23°C
4
ethanol
-
pyrazole-insensitive enzyme, pH 7.5
4
ethanol
-
wild-type enzyme Adh1
4.2
ethanol
-
isoenzyme alpha,alpha
4.6
ethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.8
ethanol
-
enzyme form ADH I
5
ethanol
-
pyrazole-insensitive enzyme, pH 10.0
5.1
ethanol
-
pH 10.5, 25°C
5.7
ethanol
-
20°C, pH 9.0
5.7
ethanol
in 100 mM Tris-HCl, at pH 5.0 and 75°C
7.2
ethanol
-
isoenzyme II from leaf
7.3
ethanol
-
isozyme ADH II, pH 8.5, 25°C
7.75
ethanol
in 50 mM Tris-HCl, pH 8.0 at 25°C
8.1
ethanol
-
enzyme form ADH-3
8.56
ethanol
-
30°C, pH 9.0
9
ethanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
9.03
ethanol
-
10°C, pH 9.0
10.4
ethanol
-
pH 9.0, 60°C, recombinant enzyme
10.6
ethanol
-
recombinant allozyme Val308, pH 7.5, 25°C
12
ethanol
-
pH 7.5, 25°C, Co-ADH
12.1
ethanol
-
40°C, pH 9.0
13
ethanol
-
in presence of 0.5 mM SDS
13.4
ethanol
-
mutant enzyme W54L
13.7
ethanol
pH 8.0, 60°C
14
ethanol
-
in presence of 3 mM SDS
17
ethanol
-
in presence of 1 mM SDS
17
ethanol
pH 7.0, temperature not specified in the publication
22
ethanol
-
mutant enzyme ADH1S-1108
24
ethanol
-
wild-type enzyme ADH1-1S
24.2
ethanol
-
pH 10.0, 50°C
26
ethanol
pH 7.0, temperature not specified in the publication
27
ethanol
-
enzyme form ADH II
27.5
ethanol
-
isoenzyme I from leaf
28
ethanol
-
isoenzyme TT-ADH
31
ethanol
pH 11.0, temperature not specified in the publication
33
ethanol
isozyme ADH2, at 21-23°C
36
ethanol
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
39
ethanol
-
pH 7.5, 25°C, Cu-ADH
39.7
ethanol
pH 7.0, temperature not specified in the publication
41
ethanol
-
in presence of 15 mM SDS
49.5
ethanol
pH 7.0, temperature not specified in the publication
50
ethanol
-
in presence of 50 mM SDS
53
ethanol
-
pH 7.5, 25°C, native Zn-ADH
64
ethanol
Sporotrichum pulverulentum
-
-
66
ethanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
120
ethanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
184
ethanol
-
carboxymethylated enzyme, at 55°C
255
ethanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
340
ethanol
-
isoenzyme ADH-1, pH 10.0
460
ethanol
-
pH 7.0, 30°C
460
ethanol
-
pH 7.0, 30°C
1625
ethanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
5000
ethanol
-
isoenzyme ADH-1, pH 7.5
1
ethyl benzoylformate
-
65°C
4.2
ethyl benzoylformate
65°C, pH 5.0
5.3
ethyl benzoylformate
65°C, pH 5.5
50
ethylene glycol
-
isoenzyme alpha,gamma1
200
ethylene glycol
-
above
290
ethylene glycol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
4.46
formaldehyde
-
-
37.65
formaldehyde
-
pH 7.0, 30°C
50
formaldehyde
Megalodesulfovibrio gigas
-
-
0.13
furfural
-
mutant S110P/Y295C, pH 6.7, 30°C
18.8
furfural
-
wild-type, pH 6.7, 30°C
42.74
furfural
-
pH 7.0, 30°C
97.37
furfural
pH 7.0, 30°C, with NADH
2.58
glycerol
-
-
16.11
glycolaldehyde
pH 7.0, 30°C, with NADH
88.32
glycolaldehyde
-
pH 7.0, 30°C
0.0007
Hexanol
-
-
0.006
Hexanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
0.085
Hexanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
0.63
Hexanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
1.9
Hexanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
6.5
Hexanol
-
mutant enzyme W54L
13.8
Hexanol
-
wilde-type enzyme ADh1
0.71
isatin
pH 5.5, 65°C
1.26
iso-propanol
pH 7.5, 23°C
2.4
iso-propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
0.26
Isobutyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.33
Isobutyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.76
Isobutyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
9
Isopropanol
-
-
20.3
Isopropanol
at pH 7.0 and 25°C
0.78
methanol
-
-
3.04
methanol
-
33°C, pH 8
12
methanol
-
pyrazole-sensitive enzyme, pH 7.5
14
methanol
-
pyrazole-sensitive enzyme, pH 10.0
150
methanol
-
isoenzyme alpha,gamma1
294.23
methanol
pH 7.5, 23°C
2.8
methyl o-chlorobenzoylformate
pH 5.5, 65°C
2.8
methyl o-chlorobenzoylformate
65°C, pH 5.5
0.13
N-benzyl-3-pyrrolidinone
-
pH 6.0
0.58
N-benzyl-3-pyrrolidinone
-
-
0.03
n-butanol
-
isoenzyme BB-ADH
0.07
n-butanol
-
isoenzyme AA-ADH
0.611
n-butanol
wild-type, pH 8.0, 60°C
2.6
n-butanol
-
isoenzyme TT-ADH
35.1
n-butanol
-
33°C, pH 8
0.02
n-Hexanol
-
isoenzyme TT-ADH
0.04
n-Hexanol
-
isoenzyme BB-ADH
0.26
n-Hexanol
-
isoenzyme TT-ADH
0.19
n-Propanol
2 mM zinc sulfate in 100 mM glycine-NaOH (pH 10.5) at 65°C
7.49
n-Propanol
-
33°C, pH 8
0.001
NAD+
pH 8.0, 60°C
0.0074
NAD+
-
isoenzyme beta1,beta1
0.0074
NAD+
-
isoenzyme beta2,beta2
0.0079
NAD+
-
isoenzyme gamma1,gamma1
0.0087
NAD+
-
isoenzyme gamma2,gamma2
0.011
NAD+
-
oxidation of ethanol
0.013
NAD+
-
isoenzyme alpha,alpha
0.02
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
0.028
NAD+
-
oxidation of propan-2-ol
0.037
NAD+
-
wild-type enzyme ADH1-1S
0.038
NAD+
-
mutant enzyme ADH1-1S1108
0.04
NAD+
-
isoenzyme ADH-2, pH 10.0 and pH 7.5
0.04
NAD+
-
isozyme ADH II, pH 8.5, 25°C
0.05
NAD+
-
isoenzyme ADH-1, pH 7.5
0.05
NAD+
-
pH 9.0, 60°C, recombinant enzyme
0.063
NAD+
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.063
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
0.073
NAD+
-
enzyme form ADH I
0.074
NAD+
Megalodesulfovibrio gigas
-
oxidation of ethanol
0.074
NAD+
-
recombinant allozyme Val308, pH 7.5, 25°C
0.089
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.097
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsC
0.1
NAD+
-
isoenzyme ADH-3
0.1
NAD+
at pH 9.0 in bis-Tris propane buffer
0.1
NAD+
-
pH 8.8, 45°C, mutant enzyme G211S
0.11
NAD+
-
enzyme form ADH II
0.12
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsA
0.127
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.13
NAD+
in 200 mM bicine, pH 9.0, at 60°C
0.17
NAD+
Sporotrichum pulverulentum
-
-
0.18
NAD+
-
isoenzyme beta1,beta1
0.2
NAD+
-
isoenzyme ADH-1, pH 7.5
0.2
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.2
NAD+
-
native enzyme, at 55°C
0.2
NAD+
mutant W87A, pH 7.0, 30°C
0.23
NAD+
-
oxidation of ethanol
0.23
NAD+
-
pH 8.8, 45°C, wild-type enzyme
0.239
NAD+
wild-type, pH 8.0, 60°C
0.24
NAD+
-
pH 10.5, 65°C
0.25
NAD+
-
isoenzyme ADH-1, pH 10.0
0.29
NAD+
pH 11.0, temperature not specified in the publication
0.29
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsG
0.39
NAD+
at pH 7.0 and 25°C
0.46
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
0.48
NAD+
-
pH 8.8, 45°C, mutant enzyme G211C
0.5
NAD+
-
carboxymethylated enzyme, at 55°C
0.5
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
0.5
NAD+
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.5
NAD+
mutant A25Y, pH 7.0, 30°C
0.5
NAD+
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
0.9
NAD+
-
reduction of ethanol
0.9
NAD+
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
1
NAD+
mutant Y25A, 30°C, pH not specified in the publication
1
NAD+
mutant Y25A, pH 7.0, 30°C
1.1
NAD+
wild-type, pH 7.0, 30°C
1.1
NAD+
wild-type, 30°C, pH not specified in the publication
1.2
NAD+
-
isozyme ADH I, pH 8.5, 25°C
1.3
NAD+
mutant W49F/W87F, 30°C, pH not specified in the publication
1.51
NAD+
cosubstrate ethanol, pH 8, 60°C
1.6
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsS
1.65
NAD+
-
pH 10.5, 25°C
1.8
NAD+
mutant W49F/W167Y, 30°C, pH not specified in the publication
4
NAD+
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
7.4
NAD+
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
8.3
NAD+
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
9.7
NAD+
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
10
NAD+
mutant V260A, 30°C, pH not specified in the publication
10.2
NAD+
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
12.4
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
14.8
NAD+
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
30
NAD+
-
isoenzyme II from leaf
31
NAD+
-
isoenzyme III from leaf
40
NAD+
-
enzyme from liver
50
NAD+
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
60
NAD+
-
enzyme from caecum
73
NAD+
-
isoenzyme I from leaf
180
NAD+
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
550
NAD+
-
reduction of propan-2-ol
710
NAD+
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
0.0004
NADH
pH 8.0, 60°C
0.0017
NADH
-
isoenzyme ADH-2, pH 7.5
0.0035
NADH
-
pH 10.5, 65°C
0.0051
NADH
-
reduction of octanal
0.0064
NADH
-
isoenzyme beta1,beta1
0.007
NADH
-
isoenzyme gamma1,gamma1
0.008
NADH
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.01
NADH
in 200 mM bicine, pH 6.0, at 60°C
0.01
NADH
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.011
NADH
-
isoenzyme alpha,alpha
0.012
NADH
-
enzyme form ADH II
0.013
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
0.016
NADH
-
pH 7.7, 60°C
0.02
NADH
Megalodesulfovibrio gigas
-
reduction of acetaldehyde
0.021
NADH
Sporotrichum pulverulentum
-
-
0.027
NADH
-
enzyme form ADH I
0.03
NADH
-
isozyme ADH II, pH 6.0, 25°C
0.03
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
0.03
NADH
-
native enzyme, at 55°C
0.038
NADH
-
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.04
NADH
-
carboxymethylated enzyme, at 55°C
0.042
NADH
-
mutant enzyme ADH1-1S1108
0.048
NADH
-
25°C, pH 7.0
0.05
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
0.062
NADH
-
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
0.071
NADH
pH 6.0, temperature not specified in the publication
0.072
NADH
wild-type, pH 8.0, 60°C
0.085
NADH
-
wild-type enzyme ADH1-1S
0.09
NADH
cosubstrate butanal, pH 7.0, 30°C
0.105
NADH
-
isoenzyme beta2,beta2
0.105
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.113
NADH
cosubstrate acetoin, pH 6.0, 70°C
0.19
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
0.2
NADH
-
isozyme ADH I, pH 5.5, 25°C
0.2
NADH
-
pH 8.0, 60°C, recombinant enzyme
0.21
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
0.75
NADH
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.4
NADH
cosubstrate acetaldehyde, pH 8, 60°C
6.4
NADH
-
isoenzyme beta1,beta1, 0.1 M sodium phosphate buffer, pH 7.5, at 25 °C
25
NADH
-
isoenzyme III from leaf
70
NADH
-
isoenzyme II from leaf
105
NADH
-
isoenzyme beta2,beta2, 0.1 M sodium phosphate buffer, pH 7.5, 25°C
121
NADH
-
isoenzyme I from leaf
260
NADH
-
isoenzyme beta3,beta3, 0.1 M sodium phospahte buffer, pH 7.5, 25°C
0.0051
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
0.066
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
0.078
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
0.113
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
0.28
NADP+
cosubstrate ethanol, pH 8, 60°C
7.4
NADP+
-
pH 8.8, 45°C, wild-type enzyme
15
NADP+
-
pH 8.8, 45°C, mutant enzyme G211S
25
NADP+
-
pH 8.8, 45°C, mutant enzyme G211C
33
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsG
66
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsS
120
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsC
360
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsA
0.033
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
0.28
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
0.043
octanal
-
enzyme form ADH-2
0.3
octanal
-
isoenzyme ADH-1, pH 7.5
3.5
octanal
-
isoenzyme ADH-1, pH 7.5
9.6
octanal
isozyme ADH3, at 21-23°C
0.0014
Octanol
-
-
0.007
Octanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
0.009
Octanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.016
Octanol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.08
Octanol
-
isoenzyme alpha,gamma1
0.6
Octanol
pH and temperature not specified in the publication
1.6
Octanol
-
isoenzyme ADH-2, pH 7.5
2.9
p-Methoxybenzaldehyde
-
isozyme ADH1, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
3.4
p-Methoxybenzaldehyde
in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
5.9
p-Methoxybenzaldehyde
-
isozyme ADH2, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
6.9
p-methoxybenzyl alcohol
-
isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
11
p-methoxybenzyl alcohol
-
isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
22
p-methoxybenzyl alcohol
in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C
0.2
pentan-1-ol
-
enzyme form ADH-3
0.012
Pentanol
-
pyrazole-sensitive enzyme, pH 7.5
0.018
Pentanol
-
pyrazole-sensitive enzyme, pH 10.0
0.09
Pentanol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
11.8
Pentanol
-
mutant enzyme W54L
56.2
Pentanol
-
wilde-type enzyme ADh1
0.19
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.26
phenylacetaldehyde
-
-
0.33
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.91
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
24.45
phenylglyoxylic acid
-
soluble enzyme
37.55
phenylglyoxylic acid
-
enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde
0.019
propan-1-ol
-
-
0.28
propan-1-ol
Megalodesulfovibrio gigas
-
-
0.5
propan-2-ol
-
-
64
propan-2-ol
Sporotrichum pulverulentum
-
-
193
propan-2-ol
-
mutant enzyme W54L
0.59
propanal
-
33°C, pH 7
41.4
propanal
-
pH 7.0, 30°C
0.91
Propanol
-
-
13.6
Propanol
-
wilde-type enzyme ADh1
43.9
Propanol
-
mutant enzyme W54L
0.011
retinol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.012
retinol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.009
trans-cinnamaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.09
trans-cinnamaldehyde
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.23
trans-cinnamaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.17
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
0.28
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
2
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.034
Vanillyl alcohol
-
0.1 M glycine-NaOH buffer, pH 10.0, 25°C
additional information
additional information
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additional information
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kinetics
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additional information
additional information
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kinetics
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additional information
additional information
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pH-dependence of Km-value
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additional information
additional information
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kinetics of ethanol oxidation
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additional information
additional information
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kinetics of ethanol oxidation
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additional information
additional information
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kinetics of native and modified enzyme with coenzyme analogues
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additional information
additional information
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Km-values of active-site Co(II)substituted enzyme
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additional information
additional information
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Km values for the class I isoenzymes with the substrates ethanol, methanol, ethylene glycol, benzyl alcohol, octanol, cyclohexanol and 16-hydroxyhexadecanoic acid
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additional information
additional information
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steady-state kinetics
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additional information
additional information
detailed kinetic mechanism, steady-state kinetics for wild-type and mutant enzymes, investigation of pH-dependency
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additional information
additional information
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detailed kinetic mechanism, steady-state kinetics for wild-type and mutant enzymes, investigation of pH-dependency
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additional information
additional information
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detailed kinetics, computational analysis of the reaction mechanism
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additional information
additional information
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Km for isozymes ADH1, and ADH4 for all retinoid substrates in forward and reverse reaction
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additional information
additional information
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Km for isozymes ADH1B1, ADH1B2, and ADH4 for all retinoid substrates in forward and reverse reaction
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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steady-state kinetics, kinetic mechanism
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additional information
additional information
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steady-state kinetics, MW 25 kDa
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additional information
additional information
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wild-type and mutant forms of the 3 isozymes, steady-state kinetics, detailed kinetic analysis, at different pH values and temperatures
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additional information
additional information
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effects of tert-butanol, butyramide, valeramide and capronamide on KM-value for ethanol
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additional information
additional information
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kinetic data füor wild-type enzyme and chimeric enzyme created by insertion of an RTX domain from the adenylate cyclase of Bordetella pertussis into a loop near the catalytic active site of the thermostable alcohol dehydrogenase D (AdhD) from Pyrococcus furiosus
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additional information
additional information
models for the Michaelis complexes with NAD+-alcohol and NADH-aldehyde are proposed
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additional information
additional information
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pre-steady-state and Michaelis-Menten steady-state kinetics, molecular dynamics simulations of enzyme-substrate interactions in the Michaelis complexes of wild-type ADH-A and Y294F/W295A double mutant. Interdependency between substrate/product and the cofactor in the ternary complex is determined, which directly affects the NADH dissociation rates, therefore, this substrate-coenzyme crosstalk plays a direct role in determining the turnover rates. Model of the kinetic mechanism of ADH-A, overview
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additional information
additional information
reaction kinetics at different pH values, overview
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