EC Number |
Protein Variants |
Reference |
---|
3.6.4.B8 | D125A |
site-directed mutagenesis of subunit ORC5 at the ATP-binding site |
756573 |
3.6.4.B8 | D159A |
site-directed mutagenesis of subunit ORC4 at the ATP-binding site. The mutation of the ORC4 Walker-B motif has little effect on ATPase activity |
756573 |
3.6.4.B8 | D620A |
site-directed mutagenesis of subunit ORC1 at the ATP-binding site. Disrupting the ORC1 Walker-B motif effectively abolishes ATPase activity |
756573 |
3.6.4.B8 | D620A/D159A |
site-directed mutagenesis of subunits ORC1 and ORC4 at the ATP-binding site. The double mutation of the Walker-B motif of both ORC1 and ORC4 abolishes activity |
756573 |
3.6.4.B8 | more |
deletion of its N-terminal DNA binding domain does not affect the activity of the wild-type RFC complex, leading to generate a truncated RFC1DELTA555 construct, that is easier to purify in the RFC pentamer |
757439 |
3.6.4.B8 | more |
mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity |
724844 |
3.6.4.B8 | more |
site-directed mutagenesis in the Walker A and SRC motifs is performed to examine the contribution of each subunit to the function of the Methanosarcina acetivorans clamp loader. Mutations in the large subunit MacRFCL and the small subunit MacRFCS2 do not impair clamp loading activity. Any mutant clamp loader harboring a mutation in MacRFCS1 is devoid of the clamp loading property |
-, 725368 |
3.6.4.B8 | R261Q |
site-directed mutagenesis of subunit ORC3 at the ATP-binding site |
756573 |
3.6.4.B8 | R69V |
site-directed mutagenesis of subunit ORC4 at the ATP-binding site |
756573 |
3.6.4.B8 | R720Q |
site-directed mutagenesis of subunit ORC1 at the ATP-binding site. The mutation abolishes ATPase activity of the motor module, and this mutation exists in a single heterozygous individual with a wild-type allele |
756573 |