3.4.22.65 | recombinant Der p 7-MBP fusion protein, mixing of 0.001 ml of 27 mg/mL protein in 25 mM HEPES, pH 7.4, 75 mM NaCl, and 5 mM maltose, with 00.001 ml of reservoir solution containing 42.5 mM Tris pH 8.5, 12.75% PEG 4000, 85 mM lithium sulfate, and 7.5% glycerol, 4°C, 1 week, X-ray diffraction structure determination and analysis at 2.35 A resolution, molecular replacement |