EC Number   |
|---|
    1.1.1.40 | - |
| 1.1.1.40 | 10 mg/ml purified enzyme in quarternary complex with NADP+, Mn2+, and oxalate, closed enzyme form, hanging drop vapour diffusion method, 4°C, from 20 mM Tris, pH 7.4, 10 mM oxalate, 5 mM MnCl2, 0.23 mM NADP+, and 2 mM DTT, with a reservoir solution containing 100 mM sodium citrate, pH 5.5, 8% PEG 6000, and 1 M LiCl, 3-4 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, atomic model |
| 1.1.1.40 | hanging drop vapor diffusion method, using 0.2 M potassium fluoride and 30% (w/v) PEG 3350 |
| 1.1.1.40 | hanging drop vapor diffusion method, using 250 mM LiCl and 19% (w/v) PEG 3350 |
| 1.1.1.40 | purified apoenzyme, hanging drop vapor diffusion method, 6 mg/ml protein is crystallized against a reservoir solution containing 250 mM LiCl and 19% w/v PEG 3350, room temperature, 5-7 days, X-ray diffraction structure determination and analysis at 2.55 A resolution, molecular replacement using the structure of pigeon c-NADP-ME in complex with NADP+, Mn2+, and oxalate as search model, modelling |
| 1.1.1.40 | recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography |
| 1.1.1.40 | sitting drop vapor diffusion method, using 0.1 M potassium chloride, 0.1 M sodium citrate (pH 5.5) and 34% (w/v) pentaerythritol propoxylate |
