EC Number   |
|---|
   3.5.1.49 | - |
| 3.5.1.49 | Escherichia coli strain Rosetta (DE3), 20-residue His-tag at N-terminus |
| 3.5.1.49 | expressed in Escherichia coli BL21 Star (DE3) cells |
| 3.5.1.49 | expressed in Escherichia coli BL21(DE3) cells |
| 3.5.1.49 | expression in Escherichia coli |
| 3.5.1.49 | gene amiF, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 3.5.1.49 | into the SalI/NotI sites of the Vector pGEX-4T-3, fused to glutathione S-transferase (GST) |
| 3.5.1.49 | the native soluble protein is expressed in Escherichia coli Rosetta (DE3) cells, the SeMet-substituted protein is expressed in the methionine-auxotrophic Escherichia coli strain B834 (DE3) |
