EC Number   |
|---|
   3.4.22.B5 | - |
| 3.4.22.B5 | expressed in Escherichia coli |
| 3.4.22.B5 | expression in Escherichia coli |
| 3.4.22.B5 | expression of CPB2.3DELTACTE (truncated CPB2.3 lacking the C-terminal extension) in Escherichia coli |
| 3.4.22.B5 | in Escherichia coli |
| 3.4.22.B5 | overexpression of CPB2.8 as an inactive pro-form lacking the characteristic C-terminal extension, CPB2.8DELTACTE in Escherichia coli. Pro-region processing is initiated during protein refolding and proceeds through several intermediate stages. Maximum enzyme activity after removal of the entire pro-region. This is facilitated by acidification |
| 3.4.22.B5 | recombinant expression of the amastigote-specific isoform CPB2.8, expressed without the C-terminal extension, termed CPB2.8DEKTACTE |
| 3.4.22.B5 | subcloned into pET-23a for expression in Escherichia coli BL21 (DE3) pLysS cells |
