EC Number   |
|---|
   3.4.22.65 | - |
| 3.4.22.65 | construction of an isopropyl-beta-D-thiogalactopyranoside-inducible expression plasmid to produce the pro-form of Der f1 in Escherichia coli |
| 3.4.22.65 | Der p 1.0124 (according to the IUIS-WHO Allergen Nomenclature Subcommittee) |
| 3.4.22.65 | DNA and amino acid sequence determination and analysis of the cDNA sequence encoding proenzyme pro-Der f1, recombinant expression od Strep-tagged proenzyme in Escherichia coli in inclusion bodies from vector pET-44a |
| 3.4.22.65 | DNA fragment encoding precursor-type recombinant Der f1 E(-1)K, which has the C-terminal amino acid of the pro-sequence, Glu, changed to Lys, expression in Aspergillus oryzae |
| 3.4.22.65 | expressed in Pichia pastoris |
| 3.4.22.65 | expressed in Saccharomyces cerevisiae |
| 3.4.22.65 | expression and maturation of prepro-Der p 1 and its mutants in Pichia pastoris. Maturation systems using Pro-Der p 1 without the prodomain glycosylation are useful for the efficient preparation of a recombinant mature allergen |
| 3.4.22.65 | expression in Escherichia coli |
| 3.4.22.65 | expression in Pichia pastoris |
