EC Number |
---|
3.4.22.65 | - |
3.4.22.65 | affinity chromatography |
3.4.22.65 | ammonium sulfate precipitation, gel filtration, ion exchange chromatography |
3.4.22.65 | anion exchange and gel filtration |
3.4.22.65 | column chromatography |
3.4.22.65 | culture supernatants recovered, buffer exchange by gel filtration, equilibration with 100 mM sodium acetate buffer (pH 4) |
3.4.22.65 | dialysis, cationic exchange on SP-Sepharose, recombinant Der p 1 dialysis, benzamidine column, dialysis |
3.4.22.65 | immunoaffinity chromatography using immobilized 4C1 anti-Der p 1 monoclonal antibody |
3.4.22.65 | mature form purified by anion exchange and gel filtration chromatography |
3.4.22.65 | mature forms were purified by anion-exchange and gel filtration chromatography, proforms were purified by means of the same methods as the mature forms but without activation by dialysis against acidic buffer, natural Der f 1 was purified from whole culture extracts of Dermatophagoides farinae |