1.1.1.188 prostaglandin-F synthase epimastigote - -, 761141 1.1.1.2 alcohol dehydrogenase (NADP+) epimastigote - 286193 1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) epimastigote - -, 712042 1.1.1.42 isocitrate dehydrogenase (NADP+) epimastigote - 723129 1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) epimastigote - 656751 1.11.1.24 thioredoxin-dependent peroxiredoxin epimastigote - 684996 1.14.14.154 sterol 14alpha-demethylase epimastigote - 701584 1.14.19.22 acyl-lipid omega-6 desaturase (cytochrome b5) epimastigote - 675584 1.14.19.28 sn-1 stearoyl-lipid 9-desaturase epimastigote - -, 745715 1.15.1.1 superoxide dismutase epimastigote - 705498 1.17.99.9 heme a synthase epimastigote - 759201 1.17.99.9 heme a synthase epimastigote the replicative life stages (epimastigotes and amastigotes) show higher TcCox15 levels than the infective and non-replicative stage (trypomastigotes). Two times higher level in epimastigotes than in amastigotes and five times higher level than in trypomastigotes -, 758753 1.3.1.6 fumarate reductase (NADH) epimastigote - 655266, 699012 1.3.3.12 L-galactonolactone oxidase epimastigote - 684985 1.3.98.1 dihydroorotate dehydrogenase (fumarate) epimastigote - 656747 1.5.5.2 proline dehydrogenase epimastigote TcPRODH mRNA and protein expression are strongly upregulated in the intracellular epimastigote, a stage which requires an external supply of proline -, 743635 1.6.2.4 NADPH-hemoprotein reductase epimastigote - 288556, 688895 1.6.2.4 NADPH-hemoprotein reductase epimastigote overexpression of TcCPR-B in Trypanosoma cruzi epimastigotes increases its resistance to the typical chemotherapeutic agents Nifurtimox and Benznidazole 688895 1.6.5.5 NADPH:quinone reductase epimastigote the enzyme is expressed throughout the Trypanosoma cruzi life cycle 671365 1.8.1.12 trypanothione-disulfide reductase epimastigote - -, 724552, 725064 1.8.1.4 dihydrolipoyl dehydrogenase epimastigote - 393962 1.8.4.11 peptide-methionine (S)-S-oxide reductase epimastigote - -, 712074 1.8.4.12 peptide-methionine (R)-S-oxide reductase epimastigote - -, 764710 2.3.1.255 N-terminal amino-acid Nalpha-acetyltransferase NatA epimastigote - -, 757472 2.3.1.256 N-terminal methionine Nalpha-acetyltransferase NatC epimastigote - -, 757472 2.4.2.7 adenine phosphoribosyltransferase epimastigote - -, 759538 2.4.99.18 dolichyl-diphosphooligosaccharide-protein glycotransferase epimastigote - 488498 2.5.1.58 protein farnesyltransferase epimastigote - 637511 2.5.1.59 protein geranylgeranyltransferase type I epimastigote - 688892 2.5.1.85 all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific] epimastigote - 674727 2.6.1.43 aminolevulinate transaminase epimastigote - 640064 2.7.1.1 hexokinase epimastigote - 640216, 640217, 640265 2.7.1.11 6-phosphofructokinase epimastigote - 640430 2.7.1.137 phosphatidylinositol 3-kinase epimastigote - -, 708996 2.7.1.2 glucokinase epimastigote - 688890 2.7.1.21 thymidine kinase epimastigote - 641149 2.7.1.6 galactokinase epimastigote expression of both isoforms GalK1 and GalK2 739235 2.7.1.67 1-phosphatidylinositol 4-kinase epimastigote - 739027 2.7.11.11 cAMP-dependent protein kinase epimastigote - 692602 2.7.2.3 phosphoglycerate kinase epimastigote - -, 738182, 761049 2.7.3.3 arginine kinase epimastigote - 642469, 662528, 673489 2.7.3.3 arginine kinase epimastigote activity increases continuously during the exponential phase of growth 642484 2.7.4.6 nucleoside-diphosphate kinase epimastigote - -, 692239, 738181, 761808 2.7.7.7 DNA-directed DNA polymerase epimastigote Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes 723132 2.7.9.1 pyruvate, phosphate dikinase epimastigote - 661517, 738186 2.7.9.1 pyruvate, phosphate dikinase epimastigote during growth of epimastigotes in batch culture, an increase in the level of the 75 kDa enzyme form and a decrease of the 100 kDa enzyme form are observed. Expression of the 100 kDa PPDK slightly decreases, when the culture reaches the stationary phase, but its specific activity decreases about 3.25times to an almost undetectable level, and the phosphorylated 75 kDa PPDK signal increases significantly -, 739234 2.7.9.3 selenide, water dikinase epimastigote cell culture 694071 3.1.1.32 phospholipase A1 epimastigote - 730354 3.1.1.32 phospholipase A1 epimastigote non-infective stage, low enzyme activity 682242 3.1.3.48 protein-tyrosine-phosphatase epimastigote - -, 714058 3.1.3.48 protein-tyrosine-phosphatase epimastigote within the endocytic pathway 664802 3.1.4.11 phosphoinositide phospholipase C epimastigote TcPI-PLC expression at high levels in the epimastigote and amastigote stages 652082 3.1.6.1 arylsulfatase (type I) epimastigote - 681811 3.2.1.143 poly(ADP-ribose) glycohydrolase epimastigote the enzyme is expressed in a life stage-dependant manner 732734 3.2.1.24 alpha-mannosidase epimastigote - 135960, 135970 3.2.1.51 alpha-L-fucosidase epimastigote - 655143 3.2.2.1 purine nucleosidase epimastigote - 326388 3.3.2.9 microsomal epoxide hydrolase epimastigote - 208891 3.4.11.1 leucyl aminopeptidase epimastigote - 717435 3.4.17.1 carboxypeptidase A epimastigote - 684959 3.4.17.19 Carboxypeptidase Taq epimastigote - 684959 3.4.21.104 mannan-binding lectin-associated serine protease-2 epimastigote - 709985 3.4.21.26 prolyl oligopeptidase epimastigote low enzyme expression 683369 3.4.22.51 cruzipain epimastigote - -, 638878, 638881, 638885, 638890, 667653, 732673, 753924, 755082 3.4.24.35 gelatinase B epimastigote - -, 708445 3.4.24.36 leishmanolysin epimastigote - -, 710664 3.4.25.2 HslU-HslV peptidase epimastigote HslV is localized in the kinetoplast of epimastigotes -, 719424 3.6.1.1 inorganic diphosphatase epimastigote - 734716 3.6.1.11 exopolyphosphatase epimastigote - 687628 3.6.5.2 small monomeric GTPase epimastigote - 667402, 667418 4.1.1.19 arginine decarboxylase epimastigote enzyme activity is only present in the early stage of the parasite exponential growth, between 4 and 23 h of cultivation in presence of Arg, with a maximum at 14 h and disappears completely at the mid logarithmic phase. It is never detected during the stationary phase -, 655193 4.1.1.49 phosphoenolpyruvate carboxykinase (ATP) epimastigote - -, 4626, 4631, 4636 4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase epimastigote enzyme is present and active in the two replicative parasite forms, epimastigotes and amastigotes, as well as in the nonreplicative, infective trypomastigotes -, 730104 4.4.1.5 lactoylglutathione lyase epimastigote - 678043, 692329 5.1.1.4 proline racemase epimastigote - 705499, 705500, 705596 5.2.1.8 peptidylprolyl isomerase epimastigote - 714320 5.2.1.8 peptidylprolyl isomerase epimastigote neither cell growth kinetics nor cell morphology are affected by the overexpression of Pin1 681821 5.3.1.6 ribose-5-phosphate isomerase epimastigote highest expression -, 678047 5.4.2.2 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) epimastigote - 703892 5.6.2.2 DNA topoisomerase (ATP-hydrolysing) epimastigote - -, 716381 6.3.1.2 glutamine synthetase epimastigote - -, 743570 6.3.1.9 trypanothione synthase epimastigote - -, 744910 7.2.2.3 P-type Na+ transporter epimastigote - -, 654200, 695869 7.4.2.1 ABC-type polar-amino-acid transporter epimastigote - 718559 7.6.2.2 ABC-type xenobiotic transporter epimastigote - -, 734833