EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.183 | expression in Escherichia coli | Rhodococcus sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.183 | molecular modeling of structure. Enzyme is a dimer. Ring stacking and a salt bridge derived from residues His114-Trp152 and Arg72-Glu82, respectively, are important forces for the subunit interaction | Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.183 | 0.076 | - |
L-phosphinothricin | pH 8.0, 35°C | Rhodococcus sp. | |
2.3.1.183 | 0.156 | - |
L-methionine sulfoximine | pH 8.0, 35°C | Rhodococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.183 | 44000 | - |
x * 44000, recombinant GST-fusion protein | Rhodococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.183 | Rhodococcus sp. | M1EYW6 | - |
- |
2.3.1.183 | Rhodococcus sp. YM12 | M1EYW6 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.183 | acetyl-CoA + L-methionine sulfoximine | - |
Rhodococcus sp. | ? | - |
? | |
2.3.1.183 | acetyl-CoA + L-methionine sulfoximine | - |
Rhodococcus sp. YM12 | ? | - |
? | |
2.3.1.183 | acetyl-CoA + L-phosphinothricin | - |
Rhodococcus sp. | CoA + L-N-acetylphosphinothricin | - |
? | |
2.3.1.183 | acetyl-CoA + L-phosphinothricin | - |
Rhodococcus sp. YM12 | CoA + L-N-acetylphosphinothricin | - |
? | |
2.3.1.183 | additional information | no substrate: glyphosate | Rhodococcus sp. | ? | - |
? | |
2.3.1.183 | additional information | no substrate: glyphosate | Rhodococcus sp. YM12 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.183 | ? | x * 44000, recombinant GST-fusion protein | Rhodococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.183 | 35 | - |
- |
Rhodococcus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.183 | - |
10 | more than 50% of maximum activity | Rhodococcus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.183 | 30 | - |
80 min, more than 80% residual activity | Rhodococcus sp. |
2.3.1.183 | 50 | - |
100 min, 60% residual activity | Rhodococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.183 | 0.5 | - |
L-methionine sulfoximine | pH 8.0, 35°C | Rhodococcus sp. | |
2.3.1.183 | 2.18 | - |
L-phosphinothricin | pH 8.0, 35°C | Rhodococcus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.183 | 8 | - |
- |
Rhodococcus sp. |