EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.2 | mutant and native enzymes expressed in Escherichia coli ET6017 | Trichormus azollae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.1.2 | D51A | 7% of activity in forward reaction, 4% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
6.3.1.2 | D51E | 5% of activity in forward reaction, 87% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
6.3.1.2 | D51N | 1.7% of activity in forward reaction, 2.4% of activity in reverse reaction no effect on protein structure | Trichormus azollae |
6.3.1.2 | D51R | complete loss of activity, no effect on protein structure | Trichormus azollae |
6.3.1.2 | D51S | 24% of activity in forward reaction, 15% of activity in reverse reaction, no effect on protein structure | Trichormus azollae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | L-methionine-DL-sulfoximine | MSX, complete inactivation of wild-type and mutant enzymes except D51E mutant, which shows only 10% inhibition | Trichormus azollae | |
6.3.1.2 | Phosphinothricin | PPT, complete inactivation of wild-type and mutant enzymes except D51E mutant, which shows 70% inhibition | Trichormus azollae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.05 | - |
ATP | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.05 | - |
NH4+ | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.07 | - |
ATP | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.12 | - |
ATP | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.21 | - |
ATP | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.32 | - |
L-glutamate | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.37 | - |
L-glutamate | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.55 | - |
NH4+ | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.85 | - |
L-glutamate | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.92 | - |
NH4+ | D51N mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 1.5 | - |
L-glutamate | wild type enzyme, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 5.95 | - |
NH4+ | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 10.6 | - |
ATP | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 12.45 | - |
NH4+ | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 16 | - |
L-glutamate | D51E mutant, pH 7.0, 30°C | Trichormus azollae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | Trichormus azollae | central enzyme of nitrogen metabolism | ADP + phosphate + L-glutamine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Trichormus azollae | Q9RHZ1 | cyanobacterium | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.2 | recombinant enzymes and enzyme from host strain | Trichormus azollae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | - |
Trichormus azollae | ADP + phosphate + L-glutamine | - |
r | |
6.3.1.2 | ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism | Trichormus azollae | ADP + phosphate + L-glutamine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.2 | GlnA | - |
Trichormus azollae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.33 | - |
L-glutamate | D51E mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 2.6 | - |
L-glutamate | D51A mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 13.1 | - |
L-glutamate | D51S mutant, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 42 | - |
L-glutamate | wild type enzyme, pH 7.0, 30°C | Trichormus azollae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.0002 | - |
L-methionine-DL-sulfoximine | wild-type enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.0003 | - |
Phosphinothricin | wild-type enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 0.1 | - |
Phosphinothricin | D51E mutant enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae | |
6.3.1.2 | 2.94 | - |
L-methionine-DL-sulfoximine | D51E mutant enzyme, biosynthetic reaction, pH 7.0, 30°C | Trichormus azollae |