EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.2 | SA0 and SA1 expressed in Escherichia coli SE3000 | Trichormus variabilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | alanine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
6.3.1.2 | aspartate | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
6.3.1.2 | cysteine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
6.3.1.2 | glycine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
6.3.1.2 | L-methionine sulfone | inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM | Trichormus variabilis | |
6.3.1.2 | L-methionine-DL-sulfoximine | inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM | Trichormus variabilis | |
6.3.1.2 | Mn2+ | inhibitory for enzyme from strain SA0 | Trichormus variabilis | |
6.3.1.2 | serine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 0.004 | - |
NH4+ | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 0.015 | - |
NH4+ | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
6.3.1.2 | 0.1 | - |
ATP | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 0.13 | - |
ATP | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
6.3.1.2 | 0.17 | - |
ATP | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 0.21 | - |
ATP | enzyme from strain SA1, pH 7.5 | Trichormus variabilis | |
6.3.1.2 | 1.4 | - |
glutamate | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 1.4 | - |
NH4+ | enzyme from strain SA1, pH 7.5 | Trichormus variabilis | |
6.3.1.2 | 1.7 | - |
glutamate | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
6.3.1.2 | 9.2 | - |
glutamate | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 12.4 | - |
NH4+ | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
6.3.1.2 | 16.8 | - |
glutamate | enzyme from strain SA1, pH 7.5 | Trichormus variabilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Mg2+ | required for optimum activity of enzyme from strain SA0, no activation of enzyme from strain SA1 | Trichormus variabilis | |
6.3.1.2 | Mn2+ | required for optimum activity of enzyme from strain SA1, highest activity at 0.3 mM, concentration inhibitory for enzyme from SA0 | Trichormus variabilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 52000 | - |
SDS-PAGE | Trichormus variabilis |
6.3.1.2 | 53080 | - |
calculated from amino acid sequence | Trichormus variabilis |
6.3.1.2 | 625000 | - |
gel filtration | Trichormus variabilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | Trichormus variabilis | central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation | ADP + phosphate + L-glutamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Trichormus variabilis | - |
strains SA0 and SA1, SA1 carries a single amino acid mutation Y183C and is defective in ammonia assimilation below concentrations of 0.5 mM | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.2 | from both strains | Trichormus variabilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 12.1 | - |
purified enzyme from strain SA1 | Trichormus variabilis |
6.3.1.2 | 126.1 | - |
purified enzyme from strain SA0 | Trichormus variabilis |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.1.2 | -70°C, concentrated solution in 50% glycerol, 2 months, 4% loss of activity | Trichormus variabilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + glutamate + NH4+ | - |
Trichormus variabilis | ADP + phosphate + L-glutamine | - |
? | |
6.3.1.2 | ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation | Trichormus variabilis | ADP + phosphate + L-glutamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.2 | dodecamer | 12 * 52000, subunit mass by SDS-PAGE, native mass by gel filtration | Trichormus variabilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 6.8 | - |
strain SA1 | Trichormus variabilis |
6.3.1.2 | 7.5 | - |
strain SA0 | Trichormus variabilis |