Literature summary for 7.3.2.1 extracted from

Nussaume, L.; Kanno, S.; Javot, H.; Marin, E.; Pochon, N.; Ayadi, A.; Nakanishi, T.M.; Thibaud, M.C.
Phosphate import in plants: focus on the PHT1 transporters (2011), Front. Plant Sci., 2, 83.

Protein Variants

Protein Variants	Comment	Organism
additional information	pht1;1-3 mutation in Arabidopsis	Hordeum vulgare
additional information	pht1;1-3 mutation in Arabidopsis	Solanum tuberosum
additional information	pht1;1-3 mutation in Arabidopsis	Solanum lycopersicum
additional information	pht1;1-3 mutation in Arabidopsis	Oryza sativa
additional information	pht1;1-3 mutation in Arabidopsis	Medicago truncatula
additional information	pht1;1-3 mutation in Arabidopsis thaliana	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	phosphate uptake kinetics in planta using radioactive phosphate isotopes	Arabidopsis thaliana
0.0031	-	phosphate/out	pH and temperature not specified in the publication	Arabidopsis thaliana
0.0091	-	phosphate/out	isozyme HvPHT1;1, pH and temperature not specified in the publication	Hordeum vulgare
0.023	-	phosphate/out	isozyme OsPT8, pH and temperature not specified in the publication	Solanum lycopersicum
0.023	-	phosphate/out	isozyme OsPT8, pH and temperature not specified in the publication	Oryza sativa
0.097	-	phosphate/out	isozyme OsPT6, pH and temperature not specified in the publication	Solanum lycopersicum
0.097	-	phosphate/out	isozyme OsPT6, pH and temperature not specified in the publication	Oryza sativa
0.13	0.28	phosphate/out	pH and temperature not specified in the publication	Solanum tuberosum
0.192	-	phosphate/out	pH and temperature not specified in the publication	Medicago truncatula
0.385	-	phosphate/out	isozyme HvPHT1;6, pH and temperature not specified in the publication	Hordeum vulgare

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	provide additional negative charges in the vicinity of the putative ER export site (D/E-X-D/E), thereby altering the recognition of this motif and resulting in the accumulation of PHT1;1 in the endoplasmic reticulum	Arabidopsis thaliana	-	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Saccharomyces cerevisiae	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Neurospora crassa	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Hordeum vulgare	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Solanum tuberosum	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Arabidopsis thaliana	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Solanum lycopersicum	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Oryza sativa	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Medicago truncatula	5886	-
plasma membrane	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop. Both C- and N-termini are expected to be oriented inside the cell, with the protein inserted in the plasma membrane	Diversispora versiformis	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	x * 58000, about, sequence calculation	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + H2O + phosphate/out	Saccharomyces cerevisiae	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Neurospora crassa	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Hordeum vulgare	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Solanum tuberosum	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Arabidopsis thaliana	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Solanum lycopersicum	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Oryza sativa	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Medicago truncatula	-	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	Diversispora versiformis	-	ADP + phosphate + phosphate/in	-	?
additional information	Saccharomyces cerevisiae	co-existence of a high and a low affinity system	?	-	?
additional information	Neurospora crassa	co-existence of a high and a low affinity system	?	-	?
additional information	Hordeum vulgare	co-existence of a high and a low affinity system	?	-	?
additional information	Solanum tuberosum	co-existence of a high and a low affinity system	?	-	?
additional information	Arabidopsis thaliana	co-existence of a high and a low affinity system	?	-	?
additional information	Solanum lycopersicum	co-existence of a high and a low affinity system	?	-	?
additional information	Oryza sativa	co-existence of a high and a low affinity system	?	-	?
additional information	Medicago truncatula	co-existence of a high and a low affinity system	?	-	?
additional information	Diversispora versiformis	co-existence of a high and a low affinity system	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Diversispora versiformis	-	-	-
Hordeum vulgare	-	-	-
Medicago truncatula	-	-	-
Neurospora crassa	-	-	-
Oryza sativa	-	-	-
Saccharomyces cerevisiae	-	gene pho84	-
Solanum lycopersicum	-	Lycopersicon esculentum	-
Solanum tuberosum	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphory lation events occur at the C-end of PHT1 proteins when phosphate is abundant in the environment. Phosphorylation of other Arabidopsis thaliana members of the family, PHT1;4, PHT1;5; PHT1;7, and PHT1;9, at the C-end of the protein	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
anther	-	Arabidopsis thaliana	-
axillary bud	-	Arabidopsis thaliana	-
flower	-	Arabidopsis thaliana	-
flower bud	-	Arabidopsis thaliana	-
lateral root	-	Arabidopsis thaliana	-
leaf	-	Arabidopsis thaliana	-
additional information	expression patterns of the nine PHT1 transporters from Arabidopsis thaliana, overview	Arabidopsis thaliana	-
pollen	-	Arabidopsis thaliana	-
root	AtPHT1;1, AtPHT1;2, and AtPHT1;4 in the root epidermis and root hairs, alsoin the tip	Arabidopsis thaliana	-
root	MtPHT1 in the root epidermis and root hairs	Medicago truncatula	-
root	revealed the presence of LePT1 transcript in the root cap and in the external layers of the root	Solanum lycopersicum	-
root	StPT2 mainly in the root epidermis and root hairs	Solanum tuberosum	-
silique	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + H2O + phosphate/out	-	Saccharomyces cerevisiae	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Neurospora crassa	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Hordeum vulgare	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Solanum tuberosum	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Arabidopsis thaliana	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Solanum lycopersicum	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Oryza sativa	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Medicago truncatula	ADP + phosphate + phosphate/in	-	?
ATP + H2O + phosphate/out	-	Diversispora versiformis	ADP + phosphate + phosphate/in	-	?
additional information	co-existence of a high and a low affinity system	Saccharomyces cerevisiae	?	-	?
additional information	co-existence of a high and a low affinity system	Neurospora crassa	?	-	?
additional information	co-existence of a high and a low affinity system	Hordeum vulgare	?	-	?
additional information	co-existence of a high and a low affinity system	Solanum tuberosum	?	-	?
additional information	co-existence of a high and a low affinity system	Arabidopsis thaliana	?	-	?
additional information	co-existence of a high and a low affinity system	Solanum lycopersicum	?	-	?
additional information	co-existence of a high and a low affinity system	Oryza sativa	?	-	?
additional information	co-existence of a high and a low affinity system	Medicago truncatula	?	-	?
additional information	co-existence of a high and a low affinity system	Diversispora versiformis	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 58000, about, sequence calculation	Arabidopsis thaliana
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Saccharomyces cerevisiae
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Neurospora crassa
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Hordeum vulgare
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Solanum tuberosum
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Arabidopsis thaliana
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Solanum lycopersicum
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Oryza sativa
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Medicago truncatula
More	PHT1 transporters show a topology with 12 membrane-spanning domains, which are separated into two groups of six domains by a charged hydrophilic loop	Diversispora versiformis

Synonyms

Synonyms	Comment	Organism
AtPHT1;1	-	Arabidopsis thaliana
AtPHT1;2	-	Arabidopsis thaliana
AtPHT1;4	-	Arabidopsis thaliana
AtPT1	-	Arabidopsis thaliana
HvPHT1;1	-	Hordeum vulgare
HvPHT1;6	-	Hordeum vulgare
LePT1	-	Solanum lycopersicum
mTPT1	-	Medicago truncatula
OsPT6	-	Oryza sativa
OsPT8	-	Oryza sativa
Pho84	-	Saccharomyces cerevisiae
PHT1 transporter	-	Saccharomyces cerevisiae
PHT1 transporter	-	Neurospora crassa
PHT1 transporter	-	Hordeum vulgare
PHT1 transporter	-	Solanum tuberosum
PHT1 transporter	-	Arabidopsis thaliana
PHT1 transporter	-	Solanum lycopersicum
PHT1 transporter	-	Oryza sativa
PHT1 transporter	-	Medicago truncatula
PHT1 transporter	-	Diversispora versiformis
StPT2	-	Solanum tuberosum

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the PHT1 transporters family	Saccharomyces cerevisiae
evolution	the enzyme belongs to the PHT1 transporters family	Neurospora crassa
evolution	the enzyme belongs to the PHT1 transporters family	Diversispora versiformis
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Hordeum vulgare
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Solanum tuberosum
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Arabidopsis thaliana
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Solanum lycopersicum
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Oryza sativa
evolution	the enzyme belongs to the PHT1 transporters family, comparison of plant PHT1 multigenic families, overview	Medicago truncatula
malfunction	a phosphorylation-mimicking mutagenesis of PHT1;1 at Ser514 results in its accumulation in the endoplasmic reticulum	Arabidopsis thaliana
additional information	transcriptional regulaiton of the PHT1 transporters, variation of phosphate concentration in the medium promotes a rapid modulation of PHT1 transcripts, overview	Arabidopsis thaliana