Cloned (Comment) | Organism |
---|---|
wild-type and mutant proteins Y36A, D46A, Y49A, D50A, and K113A are produced in Escherichia coli | Mimivirus |
Protein Variants | Comment | Organism |
---|---|---|
D46A | mutant protein displayes only trace sealing activity | Mimivirus |
D50A | nick sealing activity is 6% of wild-type activity | Mimivirus |
K113A | mutant protein is inert | Mimivirus |
additional information | truncated version of MimiLIG (C-DELTA79), composed of residues 1-557, but lacking the C-terminal 79 amino acids that comprise the BRCT domain shows reduced specific activity in nick joining by 75fold without affecting the ligase adenylylation step. The DELTA-RCT mutant of MimiLIG is impaired in sealing at a preadenylylated nick | Mimivirus |
Y36A | mutant protein displayes only trace sealing activity | Mimivirus |
Y49A | nick sealing activity is 6% of wild-type activity | Mimivirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Mimivirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mimivirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MimiLIG is produced in Escherichia coli as a His10-Smt3 fusion and purified from a soluble bacterial lysate by Ni2+-agarose chromatography | Mimivirus |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Mimivirus |
Synonyms | Comment | Organism |
---|---|---|
MimiLIG | - |
Mimivirus |