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Literature summary for 6.5.1.2 extracted from

  • Srivastava, S.K.; Dube, D.; Kukshal, V.; Jha, A.K.; Hajela, K.; Ramachandran, R.
    NAD+-dependent DNA ligase (Rv3014c) from Mycobacterium tuberculosis: novel structure-function relationship and identification of a specific inhibitor (2007), Proteins, 69, 97-111.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information deletion of just the BRCT domain from MtuLigA results in total loss of activity in in vitro assays Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
-
Mycobacterium tuberculosis
N4-(7-chloroquinolin-4-yl)-N1,N1-diethylpentame-1,4-diamine
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00146
-
NAD+
-
Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in presence of Ca2+ MtuLigA is able to carry out the first two steps of ligation reaction that is the transfer of AMP to enzyme from NAD+ and further transfer of it to substrate to form DNA-adenylate intermediate. Ca2+ can not support nick closure activity Mycobacterium tuberculosis
Mg2+ divalent cation required, optimal concentration: 10 mM Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNV1
-
-
Mycobacterium tuberculosis H37Rv P9WNV1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Mycobacterium tuberculosis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Mycobacterium tuberculosis H37Rv AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
MtuLigA
-
Mycobacterium tuberculosis
NAD+-dependent DNA ligase
-
Mycobacterium tuberculosis
Rv3014c
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 more than 60% of maximal activity between pH 7.5 and 8.5 Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0135
-
-
Mycobacterium tuberculosis 12-(6-piperidin-1-ylhexyl)-7,12-dihydro-6H-[1]benzothiepino[5,4-b]indole
0.046
-
-
Mycobacterium tuberculosis N4-(7-chloroquinolin-4-yl)-N1,N1-diethylpentame-1,4-diamine