Protein Variants | Comment | Organism |
---|---|---|
D286E | mutant enzyme shows reduced reaction rate on both match and mismatch nicked substrates compared to wild-type enzyme | Thermus sp. |
G287A | mutant enzyme shows reduced reaction rate on both match and mismatch nicked substrates compared to wild-type enzyme. The G287A mutation has a major effect on the second step | Thermus sp. |
K291R | mutant enzyme shows reduced reaction rate on both match and mismatch nicked substrates compared to wild-type enzyme | Thermus sp. |
V289I | mutant enzyme shows reduced reaction rate on both match and mismatch nicked substrates compared to wild-type enzyme | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus sp. | - |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus sp. TAK16D | - |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Thermus sp. TAK16D | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus sp. | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus sp. TAK16D | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |