Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L15F | at 20°C mutant enzyme L15F has 20fold lower ligation activity in vitro, and its activity is reduced at 42°C, resulting in 60fold lower ligation activity than wild-type LigA | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain GR501 | - |
Escherichia coli DSM 3757 | - |
strain GR501 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
LigA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
mutant enzyme L15F has 20fold lower ligation activity in vitro than wild-type enzyme | Escherichia coli |
42 | - |
mutant enzyme L15F has 60fold lower ligation activity in vitro than wild-type enzyme | Escherichia coli |