Literature summary for 6.5.1.2 extracted from

Muerhoff, A.S.; Dawson, G.J.; Desai, S.M.
A non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8 (2004), J. Virol. Methods, 119, 171-176.

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Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus HB8	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8. The assay uses the cohesive ends of restriction endonuclease digested lambda-DNA as substrate. Separation of ligated from unligated cohesive end-containing fragments via agarose gel electrophoresis, followed by digital image analysis employing a normalization procedure to correct for artifacts of gel electrophoresis, allows the accurate determination of unit ligase activity	Thermus thermophilus HB8

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	cohesive ends of restriction endonuclease digested lambda-DNA	Thermus thermophilus HB8	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?

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Release 2023.1 (January 2023)