Literature summary for 6.5.1.2 extracted from

Lu, J.; Tong, J.; Feng, H.; Huang, J.; Afonso, C.L.; Rock, D.L.; Barany, F.; Cao, W.
Unique ligation properties of eukaryotic NAD+-dependent DNA ligase from Melanoplus sanguinipes entomopoxvirus (2004), Biochim. Biophys. Acta, 1701, 37-48.

Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Melanoplus sanguinipes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000136	-	DNA	pH 7.6, 21°C, C/G matched nick DNA substrate	Melanoplus sanguinipes
0.0058	-	NAD+	pH 7.6, 21°C	Melanoplus sanguinipes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+ required, maximal activity at 10-15 mM	Melanoplus sanguinipes
Mn2+	or Mg2+ required, maximal activity at 1 mM	Melanoplus sanguinipes

Organism

Organism	UniProt	Comment	Textmining
Melanoplus sanguinipes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Melanoplus sanguinipes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + DNA	-	Melanoplus sanguinipes	?	-	?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	the enzyme seals sticky ends efficiently, but has little activity on 1-nucleotide gap or blunt-ended DNA substrates even in presence of polyethylene glycol. MsEPV DNA ligase readily joins DNA nicks with mismatches at either side of the nick junction, except for mismatches at the nick junction containing an A base in the template strand (A/A, G/A, and C/A). The enzyme can join DNA probes on RNA templates. MsEPV DNA ligase joins mismatches at the 3' side of the nick more frequently than at the 5' side of the nick on an RNA template. No activity with ATP	Melanoplus sanguinipes	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?

Synonyms

Synonyms	Comment	Organism
MsEPV DNA ligase	-	Melanoplus sanguinipes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Melanoplus sanguinipes

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
31	45	about 70% of maximal activity at 31°C and at 45°C	Melanoplus sanguinipes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.4	-	DNA	pH 7.6, 21°C, C/G matched nick DNA substrate	Melanoplus sanguinipes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Melanoplus sanguinipes

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.7	9.7	about 70% of maximal activity at pH 7.7-9.7	Melanoplus sanguinipes

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no activity with ATP	Melanoplus sanguinipes
NAD+	-	Melanoplus sanguinipes

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Release 2023.1 (January 2023)