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Literature summary for 6.5.1.2 extracted from

  • Kaczmarek, F.S.; Zaniewski, R.P.; Gootz, T.D.; Danley, D.E.; Mansour, M.N.; Griffor, M.; Kamath, A.V.; Cronan, M.; Mueller, J.; Sun, D.; Martin, P.K.; Benton, B.; McDowell, L.; Biek, D.; Schmid, M.B.
    Cloning and functional characterization of an NAD(+)-dependent DNA ligase from Staphylococcus aureus (2001), J. Bacteriol., 183, 3016-3024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
wild type and fragments expressed in Escherichia coli GI724 Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
DELTA1-390 DNA binding, but no self adenylation activity Staphylococcus aureus
DELTA316-667 active in self adenylation but unable to bind DNA Staphylococcus aureus
additional information no ligation activity when both fragments are mixed together Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75290
-
mass spectrometry Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Staphylococcus aureus DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins from Escherichia coli Staphylococcus aureus

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Staphylococcus aureus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+ required, highest activity at 0.03 mM Staphylococcus aureus