Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | highest activity at 150 mM | Aquifex pyrophilus | |
NaCl | highest activity at 200 mM | Aquifex pyrophilus | |
NH4Cl | highest activity at 200 mM | Aquifex pyrophilus | |
potassium glutamate | highest activity at 350 mM | Aquifex pyrophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Aquifex pyrophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 40% of activity obtained with Mg2+ | Aquifex pyrophilus | |
Cu2+ | 30% of activity obtained with Mg2+ | Aquifex pyrophilus | |
K+ | highest activity below 10 mM | Thermus aquaticus | |
Mg2+ | required | Aquifex pyrophilus | |
Mn2+ | 50% of activity obtained with Mg2+ | Aquifex pyrophilus | |
Na+ | inhibitory | Thermus aquaticus | |
NH4+ | highest activity below 10 mM | Thermus aquaticus | |
Ni2+ | 10% of activity obtained with Mg2+ | Aquifex pyrophilus | |
Zn2+ | 5% of activity obtained with Mg2+ | Aquifex pyrophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
SDS-PAGE | Aquifex pyrophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus aquaticus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Escherichia coli | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Aquifex pyrophilus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex pyrophilus | Q9F150 | - |
- |
Escherichia coli | - |
- |
- |
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aquifex pyrophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Thermus aquaticus | |
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Escherichia coli | |
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Aquifex pyrophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus aquaticus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Aquifex pyrophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Thermus aquaticus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Aquifex pyrophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Escherichia coli |
50 | - |
- |
Thermus aquaticus |
65 | - |
above 65°C | Aquifex pyrophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 50 | - |
Escherichia coli |
20 | 90 | - |
Thermus aquaticus |
30 | 90 | - |
Aquifex pyrophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
50% of activity lost within 15 min | Thermus aquaticus |
95 | - |
50% of activity lost within 5 min | Escherichia coli |
95 | - |
60 min, less than 25% of activity lost | Aquifex pyrophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.6 | - |
Aquifex pyrophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Thermus aquaticus | |
NAD+ | required | Escherichia coli | |
NAD+ | required | Aquifex pyrophilus |