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Literature summary for 6.5.1.2 extracted from

  • Higgins, N.P.; Cozzarelli, N.R.
    DNA-joining enzymes: A review (1979), Methods Enzymol., 68, 50-71.
    View publication on PubMed
Search for more literature for 6.5.1.2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Escherichia coli DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide the second step is the transfer of the AMP moiety from the enzyme to the 5'-phosphoryl of the DNA substrate, recreating a diphosphate linkage and thereby preserving the high-energy bond. The third step is the nucleophilic attack of the adjacent 3'-hydroxyl group to form a phosphodiester bond and eliminate AMP Escherichia coli
a
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide mechanism, the initial step is most likely a nucleophilic attack of the epsilon-amino group of a Lys on the adenylyl phosphorus of NAD+ Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joining of the 5'-phosphoryl of a DNA chain to the 3'-hydroxyl of an RNA molecule in two ways: 1. Oligo(A) is joined to the 5'-phosphoryl of oligo(dA) on a poly(dT) template strand. 2. the enzyme can circularize poly[d(A-T)]pU in which UMP occupies the 3'-hydroxyl terminal position Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m joins oligo(dT) that is base-paired to poly(dA) or oligo(dA) base-paired to poly(dT) Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA Escherichia coli ?
-
 ?