Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 50 mM | Methanocaldococcus jannaschii | |
additional information | the effects of mismatches on joining short oligonucleotides by Methanocaldococcus jannaschii DNA ligase are fully characterized. The mismatches at the first position 5' to the nick inhibits ligation more than those at the first position 3' to the nick. The mismatches at other positions 5' to the nick (3rd to 7th sites) exhibits less inhibition on ligation. However, the introduction of a C/C mismatch at the third position 5' to the nick completely inhibits the ligation of the terminal-mismatched nick of an oligonucleotide duplex by the DNA ligase | Methanocaldococcus jannaschii | |
NaCl | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | exhibits some activation on ligation | Methanocaldococcus jannaschii | |
Mg2+ | dependent on metal ions of Mg2+ and Mn2+. Ligation activity with a 2 to 25 mM concentration, optimal Mg2+ concentration is 10 mM | Methanocaldococcus jannaschii | |
Mn2+ | dependent on metal ions of Mg2+ and Mn2+ | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57635 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57635 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | ATP is the best nucleotide cofactor for Methanocaldococcus jannaschii DNA ligase. dATP also exhibits some activation on ligation. All other nucleotide cofactors, including NAD+, NADH, UTP, CTP, GTP, dTTP, dCTP, and dGTP, play no role in ligation by the enzyme | Methanocaldococcus jannaschii | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | ATP is the best nucleotide cofactor for Methanocaldococcus jannaschii DNA ligase. dATP also exhibits some activation on ligation. All other nucleotide cofactors, including NAD+, NADH, UTP, CTP, GTP, dTTP, dCTP, and dGTP, play no role in ligation by the enzyme | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MJ0171 | locus name | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | ligation activity at temperatures ranging from 37°C to 60°C | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
20 min, about 20% loss of activity | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanocaldococcus jannaschii |
8.5 | - |
- |
Methanocaldococcus jannaschii |