Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.1 extracted from

  • Lohman, G.J.; Zhang, Y.; Zhelkovsky, A.M.; Cantor, E.J.; Evans, T.C.
    Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase (2014), Nucleic Acids Res., 42, 1831-1844.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000001
-
(single-stranded DNA splinted by RNA)m value below, pH 7.4, 25°C Chlorella virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ increases activity Chlorella virus
NaCl rate of ligation is inhibited by concentrations of more than 100 mM NaCl Chlorella virus

Organism

Organism UniProt Comment Textmining
Chlorella virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (single-stranded DNA splinted by RNA)m + (single-stranded DNA splinted by RNA)n
-
Chlorella virus AMP + diphosphate + (single-stranded DNA splinted by RNA)m+n
-
?
additional information abortive adenylylation is suppressed at low ATP concentrations (below 100 mM) and pH higher than 8, leading to increased product yields. The ligation reaction is rapid for a broad range of substrate sequences, but is relatively slower for substrates with a 5'-phosphorylated dC or dG residue on the 3' side of the ligation junction Chlorella virus ?
-
?

Synonyms

Synonyms Comment Organism
DNA ligase
-
Chlorella virus
PBCV-1 DNA ligase
-
Chlorella virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Chlorella virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
(single-stranded DNA splinted by RNA)m pH 7.4, 25°C Chlorella virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Chlorella virus