Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the zinc finger does not significantly change LigIII DNA binding affinity, nor does it abrogate the specificity of the enzyme for a nicked substrate | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | in 80 mM NaCl LigIII binds non-specifically to DNA, showing equal affinity for nicked and intact DNA, in 250 mM NaCl LigIII binds specifically to nicked DNA does not bind measurably to duplex DNA | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap nickel chelating column chromatography and Sephadex S200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase III | - |
Homo sapiens |
LigIII | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |