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Literature summary for 6.5.1.1 extracted from
- Characterization of a baculovirus-encoded ATP-dependent DNA ligase (1998), J. Virol., 72, 9142-9149.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vitro transcription/translation, expression in Escherichia coli | Lymantria dispar multiple nucleopolyhedrovirus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Lymantria dispar multiple nucleopolyhedrovirus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Lymantria dispar multiple nucleopolyhedrovirus | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lymantria dispar multiple nucleopolyhedrovirus | Q9YMV2 | most probably a type III DNA ligase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant his-tagged DNA ligase | Lymantria dispar multiple nucleopolyhedrovirus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Lymantria dispar multiple nucleopolyhedrovirus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase is able to ligate a double stranded synthetic DNA substrate containing a single nick and inefficiently ligates a 1-nucleotide gap but does not ligate a 2 nucleotide gap, it is able to ligate short complementary overhangs but not blunt-ended double-stranded DNA | Lymantria dispar multiple nucleopolyhedrovirus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Lymantria dispar multiple nucleopolyhedrovirus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LdMNPV DNA ligase | - |
Lymantria dispar multiple nucleopolyhedrovirus |
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Release 2023.1 (January 2023)
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